Dong Jia-Hong, Cheng Xiao-Fei, Yin Yue-Yan, Fang Qi, Ding Ming, Li Ting-Ting, Zhang Li-Zhen, Su Xiao-Xia, McBeath Jenifer Huang, Zhang Zhong-Kai
Yunnan Provincial Key Laboratory of Agri-Biotechnology, Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650223, People's Republic of China.
Arch Virol. 2008;153(5):855-64. doi: 10.1007/s00705-008-0054-5. Epub 2008 Mar 5.
An isolate of a new tospovirus species, causing concentric zoned ringspots on fruits and necrotic lesions on leaves of infected plants, was characterised based on particle morphology, host range and serological properties. The complete nucleotide sequences of large (L), medium (M), and small (S) RNAs of this virus were found to contain 8919, 4945, and 3279 nts respectively. The L RNA encoded the RNA-dependent RNA polymerase (RdRp) (2885 aa, 332.7 kDa). The M RNA encoded a non-structural (NSm) protein (309 aa, 34.4 kDa) and a viral glycoprotein precursor (Gn/Gc) (1122 aa, 127.4 kDa). The S RNA encoded a non-structural protein (NSs) (459 aa, 51.9 kDa) and the nucleocapsid (N) protein (278 aa, 30.6 kDa). This N protein shared amino acid identities of 80.9% with those of calla lily chlorotic spot virus. Our results suggest that the virus studied here belongs to a new tospovirus species, for which the name tomato zonate spot virus is proposed.
一种新的番茄斑萎病毒属病毒分离物，可在感染植株的果实上引起同心环状环斑，在叶片上引起坏死斑，基于其粒子形态、寄主范围和血清学特性进行了鉴定。该病毒的大（L）、中（M）、小（S）RNA的完整核苷酸序列分别为8919、4945和3279个核苷酸。L RNA编码依赖RNA的RNA聚合酶（RdRp）（2885个氨基酸，332.7 kDa）。M RNA编码一种非结构（NSm）蛋白（309个氨基酸，34.4 kDa）和一种病毒糖蛋白前体（Gn/Gc）（1122个氨基酸，127.4 kDa）。S RNA编码一种非结构蛋白（NSs）（459个氨基酸，51.9 kDa）和核衣壳（N）蛋白（278个氨基酸，30.6 kDa）。该N蛋白与马蹄莲褪绿斑点病毒的N蛋白氨基酸同源性为80.9%。我们的结果表明，这里研究的病毒属于一个新的番茄斑萎病毒属病毒种，为此提出了“番茄环纹斑点病毒”这一名称。
