使用葡聚糖凝胶G-75进行轮状病毒蚀斑试验的开发。
Development of a rotavirus plaque assay using Sephadex G-75.
作者信息
Aha P M, Sabara M I
机构信息
Praxis Biologics Inc., Rochester, NY 14623.
出版信息
J Virol Methods. 1990 Apr;28(1):25-31. doi: 10.1016/0166-0934(90)90084-s.
PMID:1693368
Abstract
A rapid, reproducible and easily performed plaque assay is described for use with a variety of rotavirus strains. Plaque formation was induced in MA-104 cells by the use of Sephadex G-75, instead of the traditional agar, and crystalline trypsin in the overlay. Formation of large, discrete, easily read plaques was noted in both human and non-human rotavirus strains.
摘要
本文描述了一种快速、可重复且易于操作的蚀斑测定法，可用于多种轮状病毒株。通过使用葡聚糖凝胶G-75而非传统的琼脂，并在覆盖物中加入结晶胰蛋白酶，在MA-104细胞中诱导蚀斑形成。在人类和非人类轮状病毒株中均观察到形成了大的、离散的、易于读取的蚀斑。
Zotero 插件