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四氯化碳诱导的大鼠肝纤维化中转化生长因子-β1及I、III、IV型前胶原转录本的细胞分布

Cellular distribution of transforming growth factor-beta 1 and procollagen types I, III, and IV transcripts in carbon tetrachloride-induced rat liver fibrosis.

作者信息

Nakatsukasa H, Nagy P, Evarts R P, Hsia C C, Marsden E, Thorgeirsson S S

机构信息

Laboratory of Experimental Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

J Clin Invest. 1990 Jun;85(6):1833-43. doi: 10.1172/JCI114643.

DOI:10.1172/JCI114643
PMID:1693377
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC296648/
Abstract

The cellular distribution and temporal expression of transcripts from transforming growth factor-beta 1 (TGF-beta 1) and procollagen alpha 1(I), alpha 1(III), and alpha 1(IV) genes were studied in carbon tetrachloride (CCl4)-induced rat liver fibrosis by using in situ hybridization technique. During the fibrotic process, TGF-beta 1 and procollagen genes were similarly and predominantly expressed in Desmin-positive perisinusoidal cells (e.g., fat-storing cells and myofibroblasts) and fibroblasts and their expression continued to be higher than those observed in control rats. These transcripts were also observed in inflammatory cells mainly granulocytes and macrophage-like cells at the early stages of liver fibrosis. The production of extracellular matrix along small blood vessels and fibrous septa coincided with the expression of these genes. Expression of TGF-beta 1 and procollagen genes were not detected in hepatocytes throughout the experiment. No significant differences in cellular distribution or time course of gene expression among procollagen alpha 1(I), alpha 1(III), and alpha 1(IV) were observed. Desmin-positive perisinusoidal cells and fibroblasts appeared to play the principal role in synthesis of collagens in CCl4-induced hepatic fibrosis. The simultaneous expression of TGF-beta 1 and procollagen genes in mesenchymal cells, including Desmin-positive perisinusoidal cells, during hepatic fibrosis suggests the possibility that TGF-beta 1 may have an important role in the production of fibrosis.

摘要

采用原位杂交技术，研究了四氯化碳（CCl4）诱导的大鼠肝纤维化过程中转化生长因子-β1（TGF-β1）及前胶原α1（I）、α1（III）和α1（IV）基因转录本的细胞分布及时间表达情况。在纤维化过程中，TGF-β1和前胶原基因在结蛋白阳性的窦周细胞（如贮脂细胞和成肌纤维细胞）和成纤维细胞中呈现相似且主要的表达，其表达持续高于正常对照大鼠。在肝纤维化早期，这些转录本也在主要为粒细胞和巨噬细胞样细胞的炎性细胞中被观察到。沿小血管和纤维间隔的细胞外基质产生与这些基因的表达一致。在整个实验过程中，未在肝细胞中检测到TGF-β1和前胶原基因的表达。在前胶原α1（I）、α1（III）和α1（IV）之间，未观察到细胞分布或基因表达时间进程的显著差异。结蛋白阳性的窦周细胞和成纤维细胞似乎在CCl4诱导的肝纤维化中胶原合成中起主要作用。肝纤维化期间，TGF-β1和前胶原基因在包括结蛋白阳性窦周细胞在内的间充质细胞中同时表达，提示TGF-β1可能在纤维化产生中起重要作用。

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2
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Science. 1983 Mar 18;219(4590):1343-5. doi: 10.1126/science.6828863.
3
Detection of mrnas in sea urchin embryos by in situ hybridization using asymmetric RNA probes.
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