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Evaluation of an rRNA-derived oligonucleotide probe for culture confirmation of Neisseria gonorrhoeae.

作者信息

Rossau R, Duhamel M, Van Dyck E, Piot P, Van Heuverswyn H

机构信息

Innogenetics N.V., Antwerp, Belgium.

出版信息

J Clin Microbiol. 1990 May;28(5):944-8. doi: 10.1128/jcm.28.5.944-948.1990.

DOI:10.1128/jcm.28.5.944-948.1990
PMID:1693630
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC267843/
Abstract

The reliability of an rRNA-derived oligonucleotide probe for Neisseria gonorrhoeae was tested with 187 N. gonorrhoeae isolates, 81 Neisseria meningitidis isolates, and several strains of other bacterial species. The probe proved to be 100% specific and 100% sensitive. N. gonorrhoeae cells could also be reliably identified in contaminated cultures with the oligonucleotide probe. The 2.6-megadalton cryptic plasmid used as a probe for N. gonorrhoeae was shown to be less sensitive, detecting 179 of 181 N. gonorrhoeae isolates.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/c04ae13627a2/jcm00053-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/640f17484a4c/jcm00053-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/b699bbacadac/jcm00053-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/a48694668d05/jcm00053-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/b928b0f6c091/jcm00053-0134-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/c04ae13627a2/jcm00053-0135-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/640f17484a4c/jcm00053-0132-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/b699bbacadac/jcm00053-0133-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/a48694668d05/jcm00053-0134-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/b928b0f6c091/jcm00053-0134-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ad40/267843/c04ae13627a2/jcm00053-0135-a.jpg

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引用本文的文献

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Genitourin Med. 1997 Aug;73(4):245-52. doi: 10.1136/sti.73.4.245.
2
Comparison of monoclonal antibody methods and a ribosomal ribonucleic acid probe test for Neisseria gonorrhoeae culture confirmation.用于淋病奈瑟菌培养确认的单克隆抗体方法与核糖体核糖核酸探针检测的比较。
Eur J Clin Microbiol Infect Dis. 1993 Mar;12(3):177-84. doi: 10.1007/BF01967108.
3
Laboratory methods in genitourinary medicine. Methods of diagnosing gonorrhoea.泌尿生殖医学中的实验室方法。淋病的诊断方法。

本文引用的文献

1
DNA hybridization technique for the detection of Neisseria gonorrhoeae in men with urethritis.用于检测尿道炎男性患者中淋病奈瑟菌的DNA杂交技术。
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Auxotypes, plasmid contents, and serovars of gonococcal strains (PPNG and non-PPNG) from Jamaica.来自牙买加的淋病菌株(产青霉素酶淋球菌和非产青霉素酶淋球菌)的菌型、质粒含量及血清型
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Homology of cryptic plasmid of Neisseria gonorrhoeae with plasmids from Neisseria meningitidis and Neisseria lactamica.
Genitourin Med. 1990 Dec;66(6):453-9. doi: 10.1136/sti.66.6.453.
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Direct identification of bacterial isolates in blood cultures by using a DNA probe.使用DNA探针直接鉴定血培养中的细菌分离株。
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Polymerase chain reaction for the detection of Neisseria gonorrhoeae in clinical samples.用于检测临床样本中淋病奈瑟菌的聚合酶链反应
J Clin Pathol. 1992 May;45(5):439-42. doi: 10.1136/jcp.45.5.439.
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Evaluation of a urease-based confirmatory enzyme-linked immunosorbent assay for diagnosis of Neisseria gonorrhoeae.基于脲酶的淋病奈瑟菌诊断确证酶联免疫吸附测定的评估
J Clin Microbiol. 1992 Aug;30(8):2181-3. doi: 10.1128/jcm.30.8.2181-2183.1992.
7
Evaluation of a fluorescent DNA hybridization assay for the detection of Neisseria gonorrhoeae.
Eur J Clin Microbiol Infect Dis. 1992 Jul;11(7):602-9. doi: 10.1007/BF01961666.
淋病奈瑟菌隐蔽质粒与脑膜炎奈瑟菌和乳酸奈瑟菌质粒的同源性。
J Clin Pathol. 1986 Oct;39(10):1119-23. doi: 10.1136/jcp.39.10.1119.
4
Construction of isogenic gonococcal strains varying in the presence of a 4.2-kilobase cryptic plasmid.
J Bacteriol. 1986 Aug;167(2):685-94. doi: 10.1128/jb.167.2.685-694.1986.
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Historical perspectives and identification of Neisseria and related species.奈瑟菌属及相关菌种的历史视角与鉴定
Clin Microbiol Rev. 1988 Oct;1(4):415-31. doi: 10.1128/CMR.1.4.415.
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Molecular characterization and comparison of plasmid content in seven different strains of Neisseria gonorrhoeae.七种不同淋病奈瑟菌菌株中质粒含量的分子特征及比较
Acta Pathol Microbiol Immunol Scand B. 1987 Feb;95(1):13-21. doi: 10.1111/j.1699-0463.1987.tb03081.x.
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Plasmid. 1985 Nov;14(3):209-16. doi: 10.1016/0147-619x(85)90004-6.
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Cryptic plasmid of Neisseria gonorrhoeae: complete nucleotide sequence and genetic organization.淋病奈瑟菌的隐蔽质粒:完整核苷酸序列及基因组织
J Bacteriol. 1985 Aug;163(2):430-8. doi: 10.1128/jb.163.2.430-438.1985.
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Evaluation of a prototype DNA probe test for the noncultural diagnosis of gonorrhea.用于淋病非培养诊断的DNA探针试验原型评估。
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