Ho B S, Feng W G, Wong B K, Egglestone S I
Department of Health Sciences, Hong Kong Polytechnic, Hunghom.
J Clin Pathol. 1992 May;45(5):439-42. doi: 10.1136/jcp.45.5.439.
To evaluate the use of a cppB gene derived polymerase chain reaction (PCR) assay for direct detection of Neisseria gonorrhoeae in clinical samples.
A PCR assay was performed on 33 N gonorrhoeae strains and 12 other Neisseria species and other normal genital flora to evaluate the specificity of the chosen cppB primers. The assay was subsequently evaluated with 52 clinical swab samples collected from China.
An amplified product of 390 base pairs (bp) was observed with all the N gonorrhoeae strains, each of these products on digestion with the restriction enzyme MspI produced two bands of 250 bp and 140 bp respectively. This set of primers did not produce any amplified product of the expected length with the other non-gonococcal strains tested. For the 52 clinical swabs, 34 were culture positive and PCR successfully detected all these positives. In addition the PCR was positive for two swabs which were culture negative but positive for N gonorrhoeae antigens when tested with the ELISA method (Gonozyme).
This PCR assay is a promising diagnostic tool for detection of gonococci directly from clinical swab samples. Further evaluation is necessary.
评估使用cppB基因衍生的聚合酶链反应（PCR）检测法直接检测临床样本中淋病奈瑟菌的情况。
对33株淋病奈瑟菌菌株以及12种其他奈瑟菌属菌种和其他正常生殖系统菌群进行PCR检测，以评估所选cppB引物的特异性。随后，用从中国收集的52份临床拭子样本对该检测法进行评估。
所有淋病奈瑟菌菌株均观察到一条390个碱基对（bp）的扩增产物，这些产物经限制性内切酶MspI消化后分别产生两条带，大小分别为250 bp和140 bp。这套引物对所检测的其他非淋球菌菌株均未产生预期长度的扩增产物。对于52份临床拭子，34份培养呈阳性，PCR成功检测出所有这些阳性样本。此外，有两份拭子培养呈阴性，但用酶联免疫吸附测定法（Gonozyme）检测淋病奈瑟菌抗原呈阳性，PCR检测也呈阳性。
这种PCR检测法是一种很有前景的诊断工具，可直接从临床拭子样本中检测淋球菌。还需要进一步评估。
