基于脲酶的淋病奈瑟菌诊断确证酶联免疫吸附测定的评估
Evaluation of a urease-based confirmatory enzyme-linked immunosorbent assay for diagnosis of Neisseria gonorrhoeae.
作者信息
Carballo M, Dillon J R, Lussier M, Milthorp P, Winston S, Brodeur B
机构信息
National Laboratory for Sexually Transmitted Diseases, Ottawa, Ontario, Canada.
出版信息
J Clin Microbiol. 1992 Aug;30(8):2181-3. doi: 10.1128/jcm.30.8.2181-2183.1992.
Abstract
A new urease-based enzyme-linked immunosorbent assay utilizing novel monoclonal antibodies was evaluated for the culture confirmation of Neisseria gonorrhoeae, with 270 isolates of N. gonorrhoeae, 56 isolates of diverse Neisseria spp., and 29 Moraxella isolates. The test was highly specific (100.00%) and sensitive (97.83%). No cross-reactions were observed with any of the Neisseria or Moraxella isolates tested. Fifty percent (3 of 6) of the false-negative results were obtained with isolates of serovar IA-4, a serovar rarely encountered in North America.
摘要
一种利用新型单克隆抗体的基于脲酶的酶联免疫吸附测定法，针对270株淋病奈瑟菌、56株不同的奈瑟菌属菌株和29株莫拉克斯氏菌分离株进行了淋病奈瑟菌培养确认评估。该检测具有高度特异性（100.00%）和敏感性（97.83%）。在所测试的任何奈瑟菌或莫拉克斯氏菌分离株中均未观察到交叉反应。50%（6株中的3株）的假阴性结果是由IA - 4血清型分离株获得的，该血清型在北美很少见。
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