Lee W H, Lee H C, Tu Y C
Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taiwan.
Zhonghua Yi Xue Za Zhi (Taipei). 1993 Apr;51(4):281-8.
The nucleolar organizer regions are loops of DNA that exist in cellular nucleolus and possess ribosomal ribonucleic acid genes. Ribonucleic acid polymerase I acts on these areas. This experience collects 52 primary human gliomas at TSGH from 1984 to 1989. These included 23 astrocytomas, 8 anaplastic astrocytomas and 21 glioblastoma multiforms. After thin cutting on slides and deparaffinizing, we stained each by the silver colloid technique. We counted the total quantity of Ag-NOR dots of over 400 tumor cells of each case and average of Ag-NOR dots per cell (+/- standard error of the mean) with X1000 oil lens. The results are 1.68 +/- 0.05, 2.00 +/- 0.10; 2.55 +/- 0.13 respectively, showing significant differences among these. The average Ag-NOR paralleled the degree of histopathological malignancy. With the average Ag-NOR of tumor cells to predict the patients prognosis, we found that the group containing patients with less than 2.00 Ag-NOR per cell had better prognosis than the group with 2.00 Ag-NOR or more. The silver colloid-staining technique is a simple, rapid and reproducible method to evaluate the malignancy of human brain gliomas and to estimate the long-term prognosis of patients.
核仁组织区是存在于细胞核仁中的DNA环，含有核糖体核糖核酸基因。核糖核酸聚合酶I作用于这些区域。本研究在1984年至1989年期间收集了台湾三军总医院的52例原发性人脑胶质瘤，其中包括23例星形细胞瘤、8例间变性星形细胞瘤和21例多形性胶质母细胞瘤。将切片薄切并脱蜡后，采用银胶体技术对每例进行染色。用1000倍油镜计数每例400多个肿瘤细胞的Ag-NOR点总数及每个细胞的Ag-NOR点平均数（±平均标准误差）。结果分别为1.68±0.05、2.00±0.10、2.55±0.13，三者之间存在显著差异。平均Ag-NOR与组织病理学恶性程度平行。用肿瘤细胞的平均Ag-NOR预测患者预后，发现每细胞Ag-NOR小于2.00的患者组预后优于Ag-NOR为2.00或更高的患者组。银胶体染色技术是一种评估人脑胶质瘤恶性程度和估计患者长期预后的简单、快速且可重复的方法。
