Lin Sung-Jan, Jee Shiou-Hwa, Kuo Chien-Jui, Wu Ruei-Jr, Lin Wei-Chou, Chen Jau-Shiuh, Liao Yi-Hua, Hsu Chih-Jung, Tsai Tsen-Fang, Chen Yang-Fang, Dong Chen-Yuan
Department of Dermatology, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan.
Opt Lett. 2006 Sep 15;31(18):2756-8. doi: 10.1364/ol.31.002756.
We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, basal cell carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei and marked peripheral palisading. In the normal dermis, SHG from dermal collagen contributes largely to the multiphoton signal. However, within the cancer stroma, SHG signals diminish and are replaced by autofluorescent signals, indicating that normal collagen structures responsible for SHG have been altered. To better delineate the cancer cells and cancer stroma from the normal dermis, a quantitative MF to SHG index is developed. We demonstrate that this index can be used to differentiate cancer cells and adjacent cancer stroma from the normal dermis. Our work shows that MF and SHG imaging can be an alternative for Mohs' surgery in the real-time guidance of the secure removal of basal cell carcinoma.
我们对人基底细胞癌样本进行了多光子荧光（MF）和二次谐波产生（SHG）成像。在真皮层中，基底细胞癌可通过大量具有相对较大细胞核且周边有明显栅栏状排列的自发荧光细胞来识别。在正常真皮层中，来自真皮胶原蛋白的SHG对多光子信号有很大贡献。然而，在癌基质内，SHG信号减弱并被自发荧光信号取代，这表明负责SHG的正常胶原蛋白结构已发生改变。为了更好地从正常真皮层中区分癌细胞和癌基质，我们开发了一种定量的MF与SHG指数。我们证明该指数可用于区分癌细胞及相邻癌基质与正常真皮层。我们的工作表明，MF和SHG成像可作为莫氏手术在实时指导安全切除基底细胞癌方面的一种替代方法。
