Department of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education (MAHE), Manipal, Karnataka, 576104, India.
Institute of Biophotonics, National Yang-Ming University, 11221, Taipei, Taiwan.
Lasers Med Sci. 2021 Aug;36(6):1161-1167. doi: 10.1007/s10103-020-03144-6. Epub 2020 Sep 18.
The complete polarization state of second harmonic (SH) light was measured and characterized by collagen type I and skeletal muscle fiber using a Stokes vector-based SHG microscope. The polarization states of the SH signal are analyzed in a pixel-by-pixel manner and displayed through two dimensional (2D) Stokes vector images. Various polarization parameters are reconstructed using Stokes values to quantify the polarization properties of SH light. Also, the measurements are extended for different input polarization states to investigate the molecular structure of second harmonic generation (SHG) active molecules such as collagen type I and myosin.
利用基于斯托克斯向量的二次谐波（SHG）显微镜，对 I 型胶原蛋白和骨骼肌纤维的二次谐波光的完全偏振态进行了测量和特征分析。通过逐像素分析 SH 信号的偏振态，并通过二维（2D）斯托克斯向量图像显示。利用斯托克斯值重建各种偏振参数，以量化 SH 光的偏振特性。此外，还扩展了测量范围以研究不同的输入偏振态，以研究具有二次谐波产生（SHG）活性的分子，如胶原蛋白 I 型和肌球蛋白的分子结构。
