Boquete Manuel, Carballás Clara, Carballada Francisco, Iraola Víctor, Carnés Jerónimo, Fernández-Caldas Enrique
Allergy Department, Hospital Xeral de Calde, Lugo, Spain.
Ann Allergy Asthma Immunol. 2006 Aug;97(2):203-8. doi: 10.1016/S1081-1206(10)60014-2.
Chortoglyphus arcuatus is frequently identified in mattress dust samples in coastal areas of northern Spain.
To establish the prevalence of positive skin test reactions to C. arcuatus and to analyze its allergenicity and cross-reactivity.
One hundred thirty-eight consecutive patients entered the study. The main referred symptoms were rhinoconjunctivitis and asthma. Skin tests were performed with extracts of Dermatophagoides pteronyssinus, Lepidoglyphus destructor, Tyrophagus putrescentiae, C. arcuatus, and Glycyphagus domesticus. Enzyme-linked immunosorbent assay inhibition experiments were conducted with the different mite species and immunoblots with serum samples from 31 sensitized patients. Conjunctival provocation tests were performed in 10 patients with C. arcuatus-positive skin test reactions and in 9 with negative results; all the patients had positive skin test reactions to D. pteronyssinus.
The prevalence of positive skin test reactions to D. pteronyssinus was 94.2% and to C. arcuatus was 58%. There was a significant correlation between the number of mites to which patients were sensitized and the time of disease evolution (P = .02). Results of enzyme-linked immunosorbent assay inhibition experiments suggested minimal cross-reactivity between C. arcuatus and D. pteronyssinus. Immunoblot results confirmed specific IgE binding to several bands. Conjunctival test results were positive in 9 of 10 C. arcuatus-positive individuals and slightly positive in 2 of 9 C. arcuatus-negative, D. pteronyssinus-positive individuals.
There is a high prevalence of sensitization to C. arcuatus in northern Spain. Sensitization to this species should be considered of clinical significance. There is minimal cross-reactivity between C. arcuatus and D. pteronyssinus.
在西班牙北部沿海地区的床垫灰尘样本中经常发现弓毛食甜螨。
确定对弓毛食甜螨皮肤试验阳性反应的患病率，并分析其变应原性和交叉反应性。
138例连续患者进入本研究。主要提及的症状为鼻结膜炎和哮喘。用尘螨、腐食酪螨、椭圆食粉螨、弓毛食甜螨和家甜食螨提取物进行皮肤试验。对不同螨种进行酶联免疫吸附试验抑制实验，并用31例致敏患者的血清样本进行免疫印迹分析。对10例弓毛食甜螨皮肤试验阳性反应患者和9例阴性结果患者进行结膜激发试验；所有患者对尘螨皮肤试验均呈阳性反应。
对尘螨皮肤试验阳性反应的患病率为94.2%，对弓毛食甜螨为58%。患者致敏螨的数量与疾病演变时间之间存在显著相关性（P = 0.02）。酶联免疫吸附试验抑制实验结果表明弓毛食甜螨与尘螨之间的交叉反应性最小。免疫印迹结果证实特异性IgE与几条条带结合。10例弓毛食甜螨阳性个体中有9例结膜试验结果为阳性，9例弓毛食甜螨阴性、尘螨阳性个体中有2例呈弱阳性。
在西班牙北部，对弓毛食甜螨致敏的患病率很高。对该螨种的致敏应被视为具有临床意义。弓毛食甜螨与尘螨之间的交叉反应性最小。
