Sun Lihong, Liu Yuqiang, Jiang Ling, Su Changchao, Wang Chunming, Zhai Huqu, Wan Jianmin
National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, PR China.
Hereditas. 2007 May;144(2):48-52. doi: 10.1111/j.2006.0018-0661.01932.x.
The brown planthopper (BPH) is one of the most serious insect pests of rice throughout Asia. In this study, we constructed a linkage map to determine the locus for BPH resistance gene, using an F(2) population from a cross between a resistant indica cultivar, 'Col.5 Thailand', and a susceptible cultivar '02428'. Insect resistance was evaluated using 147 F(3) families and the genotype of each F(2) plant was inferred from the phenotype of corresponding F(3) families. Two QTLs was detected on chromosome 2 (explains 29.4% phenotypic variation) and 6 (46.2% variation explained) associated with resistance to BPH in the mapping population. Comparison of the chromosomal locations and reactions to BPH biotypes indicated that the gene on chromosome 6 is different from at least 18 of the 19 previously identified BPH resistance genes. These two genes have large effects on BPH resistance and may be a useful BPH resistance resource for rice breeding programs.
褐飞虱是亚洲水稻最严重的害虫之一。在本研究中，我们构建了一个连锁图谱来确定抗褐飞虱基因的位点，使用的是抗性籼稻品种‘Col.5 Thailand’与感病品种‘02428’杂交产生的F(2)群体。利用147个F(3)家系评估抗虫性，并根据相应F(3)家系的表型推断每个F(2)植株的基因型。在第2号染色体上检测到两个与抗褐飞虱相关的QTL（解释29.4%的表型变异），在第6号染色体上检测到一个QTL（解释46.2%的变异）。对染色体位置和对褐飞虱生物型反应的比较表明，第6号染色体上的基因与之前鉴定的19个褐飞虱抗性基因中的至少18个不同。这两个基因对褐飞虱抗性有很大影响，可能是水稻育种计划中有用的抗褐飞虱资源。
