Mori M, Tsukitani K, Ninomiya T, Okada Y
Department of Oral Surgery, Asahi University School of Dentistry, Gifu, Japan.
Pathol Res Pract. 1987 Oct;182(5):632-46.
Variant expressions of modified myoepithelial cells in salivary pleomorphic adenomas are described as determined by immunohistochemical techniques which visualized the distributions of S-100 protein, intermediate-sized filament proteins (keratin, vimentin, and desmin), and contractile proteins (myosin and actin), as well as lysozyme and lactoferrin. Immunohistochemical staining patterns of S-100 protein were basically used to classify modified myoepithelial cells, along with histologic criteria. Histochemical modifications of myoepithelial cells in pleomorphic adenoma of salivary glands could be divided into a) reactive, b) transformed, and c) neoplastic myoepithelial cells. Reactive myoepithelial cells were stromal-like cells which displayed an intense S-100 protein reaction. Transformed myoepithelial cells were negative or slightly positive for S-100 protein; they were located in the outer zone of tubular or duct-like structures and were spindle-shaped. The inner round cells of tubular and ductal structures, which could be ductal origin, gave intense keratin staining, as well as marked reactions for lysozyme and lactoferrin. Neoplastic myoepithelial cells were plasmatoid or fibrous types of cells and contained abundant S-100 protein and vimentin. These cells were termed "myoepithelioma" as in classical diagnosis.
通过免疫组织化学技术描述了唾液多形性腺瘤中修饰的肌上皮细胞的不同表达形式，该技术可观察到S-100蛋白、中等大小的丝状蛋白（角蛋白、波形蛋白和平滑肌肌动蛋白）、收缩蛋白（肌球蛋白和肌动蛋白）以及溶菌酶和乳铁蛋白的分布。S-100蛋白的免疫组织化学染色模式基本用于与组织学标准一起对修饰的肌上皮细胞进行分类。涎腺多形性腺瘤中肌上皮细胞的组织化学改变可分为：a）反应性、b）转化性和c）肿瘤性肌上皮细胞。反应性肌上皮细胞为间质样细胞，显示强烈的S-100蛋白反应。转化性肌上皮细胞对S-100蛋白呈阴性或弱阳性；它们位于管状或导管样结构的外层，呈梭形。管状和导管结构的内部圆形细胞可能起源于导管，角蛋白染色强烈，溶菌酶和乳铁蛋白反应也明显。肿瘤性肌上皮细胞为浆细胞样或纤维样细胞，含有丰富的S-100蛋白和波形蛋白。如在经典诊断中一样，这些细胞被称为“肌上皮瘤”。
