阿霉素耐药的MCF-7乳腺癌细胞线粒体蛋白质组的改变
Alterations in the mitochondrial proteome of adriamycin resistant MCF-7 breast cancer cells.
作者信息
Strong Rachael, Nakanishi Takeo, Ross Douglas, Fenselau Catherine
机构信息
Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland, USA.
出版信息
J Proteome Res. 2006 Sep;5(9):2389-95. doi: 10.1021/pr060207c.
A 2D-gel based comparative proteomic analysis has been conducted of changes in mitochondrial protein abundances in MCF-7 human breast cancer cells selected for resistance to Adriamycin accompanied by verapamil. We identified and compared 156 unique proteins from 184 spots. Eleven mitochondrial proteins were found with abundances altered more than 2-fold. Transcription was evaluated for two of these, using quantitative RT-PCR. Implications of the changes are considered with respect to drug resistance.
已对用维拉帕米处理后对阿霉素产生抗性的MCF-7人乳腺癌细胞中线粒体蛋白质丰度的变化进行了基于二维凝胶的比较蛋白质组学分析。我们从184个斑点中鉴定并比较了156种独特的蛋白质。发现11种线粒体蛋白质的丰度变化超过2倍。使用定量RT-PCR对其中两种蛋白质的转录进行了评估。并从耐药性方面考虑了这些变化的影响。
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