Ng Sing Muk, Narayanaswamy R
School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M60 1QD, UK.
Anal Bioanal Chem. 2006 Nov;386(5):1235-44. doi: 10.1007/s00216-006-0736-3. Epub 2006 Sep 1.
This paper describes the investigation of a molecularly imprinted polymer (MIP) as a sensing receptor for Al(3+) ion detection by using an optical approach. Al(3+) ion was adopted as the template molecule and 8-hydroxyquinoline sulfonic acid ligand as the fluorescence tag. The polymer was synthesised using acrylamide as monomer, 2-hydroxyethyl methacrylate as co-monomer and ethylene glycol dimethracylate as cross-linker. The free radical polymerisation was performed in methanol and initiated by 2,2'-azobisisobutyronitrile at 70 degrees C. The imprinted polymer was fluorometrically characterised using a fibre optic attachment in a self-designed flow-cell. NaF was used to leach the Al(3+) ion from the MIP. The optimum pH for the rebinding of Al(3+) ion with the leached polymer was found to be pH 5 and the fluorescence response was found to be stable within the buffer strength range of 0.05-0.10 M. The fluorescence intensity during Al(3+) ion rebinding was inversely dependent on temperature, and a low interference response (<3%) toward metal ions except for Cu(2+) and Zn(2+) ions was observed. The polymer rebinding repeatability study conducted over 9 cycles with Al(3+) ion (0.8 x 10(-4) M) was found to give an RSD value of 2.82% with a standard deviation of 0.53. The dynamic range of the system was found to be linear up to 1.0 x 10(-4) M Al(3+) ion with a limit of detection of 3.62 microM.
本文描述了一种分子印迹聚合物（MIP）作为传感受体，通过光学方法检测Al(3+)离子的研究。采用Al(3+)离子作为模板分子，8-羟基喹啉磺酸配体作为荧光标签。以丙烯酰胺为单体、甲基丙烯酸2-羟乙酯为共聚单体、乙二醇二甲基丙烯酸酯为交联剂合成了该聚合物。自由基聚合在甲醇中进行，由2,2'-偶氮二异丁腈在70℃引发。使用自行设计的流通池中的光纤附件对印迹聚合物进行荧光表征。用NaF从MIP中浸出Al(3+)离子。发现Al(3+)离子与浸出聚合物重新结合的最佳pH值为pH 5，并且在0.05-0.10 M的缓冲强度范围内荧光响应稳定。Al(3+)离子重新结合期间的荧光强度与温度成反比，并且观察到除Cu(2+)和Zn(2+)离子外对金属离子的低干扰响应（<3%）。对Al(3+)离子（0.8×10(-4) M）进行9个循环的聚合物重新结合重复性研究，发现相对标准偏差（RSD）值为2.82%，标准偏差为0.53。该系统的动态范围在高达1.0×10(-4) M的Al(3+)离子浓度下呈线性，检测限为3.62 microM。
