中毒性休克综合征毒素1及其他葡萄球菌外毒素与人外周血单核细胞表面受体的结合竞争
Binding competition of toxic shock syndrome toxin 1 and other staphylococcal exoproteins for receptors on human peripheral blood mononuclear cells.
作者信息
See R H, Krystal G, Chow A W
机构信息
Department of Medicine, University of British Columbia, Vancouver, Canada.
出版信息
Infect Immun. 1990 Jul;58(7):2392-6. doi: 10.1128/iai.58.7.2392-2396.1990.
Abstract
Binding of toxic shock toxin 1 (TSST-1) and staphylococcal enterotoxin A (SEA) to human peripheral blood mononuclear cells (PBMC) was investigated by using 125I-labeled ligands. Scatchard analyses revealed similar numbers of receptors (approximately 5,000 to 8,000) and similar dissociation constants (Kd, approximately 20 to 25 nM) per PBMC. SEA but not enterotoxin B, C1, C2, C3, D, or E significantly inhibited binding of 125I-TSST-1 to PBMC. Cross-competition of TSST-1 and SEA in binding assays suggests that they may bind to overlapping or separate epitopes on the same receptor.
摘要
利用125I标记的配体研究了毒性休克毒素1（TSST-1）和葡萄球菌肠毒素A（SEA）与人外周血单个核细胞（PBMC）的结合情况。Scatchard分析显示，每个PBMC的受体数量相似（约5000至8000个），解离常数（Kd，约20至25 nM）也相似。SEA可显著抑制125I-TSST-1与PBMC的结合，而肠毒素B、C1、C2、C3、D或E则无此作用。TSST-1和SEA在结合试验中的交叉竞争表明，它们可能结合于同一受体上重叠或分开的表位。
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本文引用的文献
1
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J Infect Dis. 1981 Apr;143(4):509-16. doi: 10.1093/infdis/143.4.509.
2
Induction of human interleukin-1 by a product of Staphylococcus aureus associated with toxic shock syndrome.金黄色葡萄球菌一种与中毒性休克综合征相关的产物诱导人白细胞介素-1的产生。
J Clin Invest. 1984 May;73(5):1312-20. doi: 10.1172/JCI111334.
3
Staphylococcal toxic shock toxin specifically binds to cultured human epithelial cells and is rapidly internalized.葡萄球菌中毒性休克毒素特异性结合培养的人上皮细胞并迅速被内化。
Infect Immun. 1984 Sep;45(3):566-71. doi: 10.1128/iai.45.3.566-571.1984.
4
5
Binding of staphylococcal enterotoxin A (SEA) with human splenic lymphocytes.
Int J Biochem. 1983;15(3):285-8. doi: 10.1016/0020-711x(83)90092-7.
6
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Anal Biochem. 1981 Apr;112(2):195-203. doi: 10.1016/0003-2697(81)90281-5.
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