See R H, Krystal G, Chow A W
Department of Medicine, University of British Columbia, Vancouver, Canada.
Infect Immun. 1990 Jul;58(7):2392-6. doi: 10.1128/iai.58.7.2392-2396.1990.
Binding of toxic shock toxin 1 (TSST-1) and staphylococcal enterotoxin A (SEA) to human peripheral blood mononuclear cells (PBMC) was investigated by using 125I-labeled ligands. Scatchard analyses revealed similar numbers of receptors (approximately 5,000 to 8,000) and similar dissociation constants (Kd, approximately 20 to 25 nM) per PBMC. SEA but not enterotoxin B, C1, C2, C3, D, or E significantly inhibited binding of 125I-TSST-1 to PBMC. Cross-competition of TSST-1 and SEA in binding assays suggests that they may bind to overlapping or separate epitopes on the same receptor.
利用125I标记的配体研究了毒性休克毒素1（TSST-1）和葡萄球菌肠毒素A（SEA）与人外周血单个核细胞（PBMC）的结合情况。Scatchard分析显示，每个PBMC的受体数量相似（约5000至8000个），解离常数（Kd，约20至25 nM）也相似。SEA可显著抑制125I-TSST-1与PBMC的结合，而肠毒素B、C1、C2、C3、D或E则无此作用。TSST-1和SEA在结合试验中的交叉竞争表明，它们可能结合于同一受体上重叠或分开的表位。
