Anttila H S, Reitamo S, Erkko P, Miettinen A, Didierjean L, Saurat J H
Department of Dermatology, Helsinki University Central Hospital, Finland.
J Invest Dermatol. 1990 Jul;95(1):31-8. doi: 10.1111/1523-1747.ep12873278.
Previous studies have shown that interleukin-1 (IL-1) is present in normal human epidermis. However, with immunohistochemical techniques, epidermal IL-1 immunoreactivity has been found in only a limited number of epidermal cells. In the present study, we show that both IL-1 alpha and beta immunoreactivities can be detected in all epidermal cell layers, provided optimal processing of tissue samples is used. The use of isolated epidermal cells showed that keratinocytes at various stages of maturation display both membrane-associated and cytosolic IL-1 alpha and beta immunoreactivities. After protease treatment of tissue sections, the IL-1 beta immunoreactivity of the granular cell layer was enhanced by some antibodies used, whereas in the other cell layers it was clearly lower. We a) suggest a different cellular localization, processing, and/or binding to subcellular structures of IL-1 during the differentiation process of human keratinocytes and b) outline the technical difficulties in any immunohistologic approach to IL-1 status in diseased skin.
先前的研究表明，白细胞介素-1（IL-1）存在于正常人体表皮中。然而，采用免疫组织化学技术时，仅在有限数量的表皮细胞中发现了表皮IL-1免疫反应性。在本研究中，我们发现，只要对组织样本进行优化处理，在所有表皮细胞层中均可检测到IL-1α和β免疫反应性。对分离的表皮细胞进行研究发现，处于不同成熟阶段的角质形成细胞均显示出与膜相关的以及胞质内的IL-1α和β免疫反应性。对组织切片进行蛋白酶处理后，所用的一些抗体增强了颗粒细胞层的IL-1β免疫反应性，而在其他细胞层中其免疫反应性明显较低。我们a）提出在人类角质形成细胞分化过程中，IL-1在细胞定位、处理和/或与亚细胞结构结合方面存在差异，b）概述了在对患病皮肤的IL-1状态进行任何免疫组织学检测时遇到的技术难题。
