Bujia J, Kim C, Ostos-Aumente P, Lopez-Villarejo J, Kastenbauer E
Department of Otolaryngology, Head and Neck Surgery, Ludwig Maximilians University, Munich, Germany.
Eur Arch Otorhinolaryngol. 1997;254(1):6-8. doi: 10.1007/BF02630748.
Middle ear cholesteatoma epithelium is a rich source of interleukin-1-alpha (IL-1-alpha), being involved in both keratinocyte hyperproliferation and bone destruction. IL-1-alpha exerts its effects by binding to two distinct IL-1 receptors (IL-1-R). In this study, we have examined the expression of IL-1-R type II (IL-1-R-II) in cholesteatoma samples and have quantified these levels with computer-assisted image analysis. Normal aural skin was used as control. Immunostaining demonstrated the presence of IL-1-R-II in both epidermis and cholesteatoma keratinocytes. The receptors were 3 times higher than those in normal epidermis. The presence of IL-1-alpha in cholesteatoma epithelium coupled with the induced expression of IL-1-R-II indicates the existence of a highly regulated system of autocrine stimulation of cholesteatoma keratinocytes by IL-1.
中耳胆脂瘤上皮是白细胞介素-1α（IL-1α）的丰富来源，参与角质形成细胞过度增殖和骨质破坏。IL-1α通过与两种不同的IL-1受体（IL-1-R）结合发挥作用。在本研究中，我们检测了胆脂瘤样本中II型IL-1受体（IL-1-R-II）的表达，并通过计算机辅助图像分析对这些水平进行了定量。正常耳皮肤用作对照。免疫染色显示表皮和胆脂瘤角质形成细胞中均存在IL-1-R-II。这些受体比正常表皮中的受体高3倍。胆脂瘤上皮中IL-1α的存在以及IL-1-R-II的诱导表达表明存在一个由IL-1对胆脂瘤角质形成细胞进行自分泌刺激的高度调节系统。
