Kobayashi Mime, Sumitomo Koji, Torimitsu Keiichi
NTT Basic Research Laboratories, NTT Corporation, 3-1 Morinosato-Wakamiya, Atsugi, Kanagawa, 243-0198, Japan.
Ultramicroscopy. 2007 Feb-Mar;107(2-3):184-90. doi: 10.1016/j.ultramic.2006.07.008. Epub 2006 Aug 22.
The direct observation of individual molecules in action is required for a better understanding of the mechanisms of biological reactions. We used a high-speed atomic force microscope (AFM) in solution to visualize short DNA fragments in motion. The technique represents a new approach in analyzing molecular interactions, and it allowed us to observe real-time images of biotinylated DNA binding to/dissociating from streptavidin protein. Our results show that high-speed AFMs have the potential to reveal the mechanisms of molecular interactions, which cannot be determined by analyzing the average value of mass reactions.
为了更好地理解生物反应机制，需要直接观察单个分子的作用。我们在溶液中使用高速原子力显微镜（AFM）来可视化运动中的短DNA片段。该技术代表了一种分析分子相互作用的新方法，使我们能够观察生物素化DNA与链霉亲和素蛋白结合/解离的实时图像。我们的结果表明，高速原子力显微镜有潜力揭示分子相互作用的机制，而这是通过分析质量反应的平均值无法确定的。
