生物分子复合物纳米级动力学的直接原子力显微镜可视化
Direct AFM Visualization of the Nanoscale Dynamics of Biomolecular Complexes.
作者信息
Lyubchenko Yuri L
机构信息
Department of Pharmaceutical Sciences, University of Nebraska Medical Center, Omaha, NE 68198, USA.
出版信息
J Phys D Appl Phys. 2018;51(40). doi: 10.1088/1361-6463/aad898. Epub 2018 Aug 20.
Abstract
High-speed AFM (HS-AFM) is an advanced technique with numerous applications in biology, particularly in molecular biophysics. Developed as a time-lapse AFM technique for direct imaging fully hydrated biological molecules, HS-AFM is currently capable of visualizing the dynamics of biological molecules and their complexes at a video-data acquisition rate. Spatial resolution at the nanometer level is another important characteristic of HS-AFM. This review focuses on examples of primarily protein-DNA complexes to illustrate the high temporal and spatial resolution capabilities of HS-AFM that have resulted in novel models and/or the functional mechanisms of these biological systems.
摘要
高速原子力显微镜（HS-AFM）是一项先进技术，在生物学领域，尤其是分子生物物理学中有众多应用。作为一种用于直接成像完全水合生物分子的延时原子力显微镜技术，HS-AFM目前能够以视频数据采集速率可视化生物分子及其复合物的动态变化。纳米级的空间分辨率是HS-AFM的另一重要特性。本综述聚焦于主要蛋白质-DNA复合物的实例，以阐明HS-AFM的高时间和空间分辨率能力，这些能力已带来了这些生物系统的新模型和/或功能机制。
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