DiSilvio Lucy, Jameson Jacqueline, Gamie Zakareya, Giannoudis Peter V, Tsiridis Eleftherios
Department of Biomaterial and Biomimetics, Guy's, King's and St Thomas' Dental Institute, University of London, London SE1 1UL, UK.
Injury. 2006 Sep;37 Suppl 3:S33-42. doi: 10.1016/j.injury.2006.08.022.
Estrogen may increase the proliferation of osteoblasts depending upon their differentiation stage. Our objective was to test the hypothesis that estradiol could stimulate the proliferation of primary human osteoblast (HOB) cells or human mesenchymal stem cells (h-MSCs). To test this hypothesis, we investigated two synthetic estradiol preparations: (a) a commercially available one (in clinical use) whose effect was evaluated using MTT assay, trypan blue cell counts and total protein assays; (b) a novel synthetic preparation (not in clinical use) using Alamar Blue assays and scanning electron microscopy (SEM). Commercial estradiol increased HOB proliferation in a concentration-dependant manner between 1 nM and 8 nM reaching significance at a concentration of 8 nM (p<0.001). Small doses of 1 nM and 2 nM increased h-MSC proliferation (p<0.001) but higher doses had no effect. All novel synthetic estradiol preparations decreased HOB proliferation (p<0.001) whereas no direct effect was observed on h-MSCs. Commercial estradiol appears to induce proliferation of HOBs, although its effects on h-MSCs appears to be highly dose-dependent and requires further investigation.
雌激素可能会根据成骨细胞的分化阶段增加其增殖。我们的目的是检验雌二醇能否刺激原代人成骨细胞（HOB）或人间充质干细胞（h-MSC）增殖这一假设。为验证该假设，我们研究了两种合成雌二醇制剂：（a）一种市售制剂（用于临床），其效果通过MTT法、台盼蓝细胞计数和总蛋白测定进行评估；（b）一种新型合成制剂（未用于临床），采用alamar蓝法和扫描电子显微镜（SEM）进行研究。市售雌二醇在1 nM至8 nM浓度范围内以浓度依赖方式增加HOB增殖，在8 nM浓度时达到显著水平（p<0.001）。小剂量的1 nM和2 nM增加h-MSC增殖（p<0.001），但更高剂量则无作用。所有新型合成雌二醇制剂均降低HOB增殖（p<0.001），而对h-MSCs未观察到直接作用。市售雌二醇似乎能诱导HOB增殖，尽管其对h-MSCs的作用似乎高度依赖剂量，需要进一步研究。
