Paez E, Esteban M
Virology. 1984 Apr 15;134(1):12-28. doi: 10.1016/0042-6822(84)90268-x.
In this investigation the sensitivity of vaccinia virus to interferon (IFN) has been examined in cultured cells. In a variety of mouse and human cells of different origins vaccinia virus functions (RNA, protein, and virus yields) were found to be relatively resistant to IFN. In these systems, the levels of the IFN-mediated enzyme activities (2-5A synthetase and protein kinase) were severely impaired by the virus. This virus-mediated inhibitory effect developed with time after infection and was dependent on viral protein synthesis. Mixed infections between vaccinia virus and viruses (VSV or polio) which are sensitive to IFN showed that both protein synthesis and virus yields were not inhibited. These findings show that vaccinia virus can overcome the antiviral action of IFN and that viral gene functions appear to be involved in this interference phenomenon.
在本研究中，已在培养细胞中检测了痘苗病毒对干扰素（IFN）的敏感性。在多种来源不同的小鼠和人类细胞中，发现痘苗病毒的功能（RNA、蛋白质和病毒产量）对干扰素相对耐药。在这些系统中，病毒严重损害了干扰素介导的酶活性（2-5A合成酶和蛋白激酶）水平。这种病毒介导的抑制作用在感染后随时间发展，并且依赖于病毒蛋白合成。痘苗病毒与对干扰素敏感的病毒（水疱性口炎病毒或脊髓灰质炎病毒）之间的混合感染表明，蛋白质合成和病毒产量均未受到抑制。这些发现表明，痘苗病毒能够克服干扰素的抗病毒作用，并且病毒基因功能似乎参与了这种干扰现象。
