Irie Yasuhiko, Preston Andrew, Yuk Ming H
Department of Microbiology, University of Pennsylvania School of Medicine, 201C Johnson Pavilion, 3610 Hamilton Walk, Philadelphia, PA 19104-6084, USA.
J Bacteriol. 2006 Sep;188(18):6680-7. doi: 10.1128/JB.00605-06.
We previously showed that the Bvg virulence control system regulates biofilm formation in Bordetella bronchiseptica (Y. Irie, S. Mattoo, and M. H. Yuk, J. Bacteriol. 186:5692-5698, 2004). Analyses of the extracellular components of B. bronchiseptica biofilm matrix revealed that the major sugar component in the matrix was xylose, and linkage analysis indicated a majority of it to be in a 4-linked polymeric form. The production of xylose was independent of Bvg regulation but instead was dependent on bacterial growth phase. In addition, N-acetyl-glucosamine in the matrix was found to be important for the initial development of the biofilm. These results suggest that B. bronchiseptica biofilm formation is growth phase dependent in addition to being regulated by the Bvg virulence system.
我们之前的研究表明，Bvg 毒力控制系统可调节支气管败血波氏杆菌中的生物膜形成（Y. Irie、S. Mattoo 和 M. H. Yuk，《细菌学杂志》186:5692 - 5698，2004 年）。对支气管败血波氏杆菌生物膜基质的细胞外成分分析表明，基质中的主要糖类成分是木糖，并且连接分析表明其大部分以 4 - 连接的聚合物形式存在。木糖的产生不依赖于 Bvg 调节，而是依赖于细菌生长阶段。此外，发现基质中的 N - 乙酰葡糖胺对生物膜的初始形成很重要。这些结果表明，支气管败血波氏杆菌生物膜的形成除了受 Bvg 毒力系统调节外，还依赖于生长阶段。
