Söder B, Airila Månsson S, Söder P-O, Kari K, Meurman J
Institute of Odontology, Karolinska Institutet, Huddinge, Sweden.
J Periodontal Res. 2006 Oct;41(5):411-7. doi: 10.1111/j.1600-0765.2006.00888.x.
To investigate the levels of matrix metalloproteinase (MMP) -8 and -9 with the simultaneous presence of periodontal pathogens in gingival crevicular fluid (GCF) as well as MMP-9 and cholesterol in blood. Although bacterial pathogens are required to initiate the periodontal disease process, in some individuals the reaction to bacteria may lead to an excessive host response, resulting in a general inflammatory response.
MMP-9 and lipids were analyzed from the blood samples of 33 subjects with a 16-year history and oral health records of periodontal disease as well as from 31 periodontally healthy controls. Information was obtained on education, body mass index, and family history of atherosclerosis. GCF was taken to determine MMP-8 and MMP-9 levels, and bacterial samples were simultaneously collected for polymerase chain reaction assessment of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Prevotella nigrescens, Tannerella forsythia, and Treponema denticola. Analysis of variance, chi-squared test, and multiple logistic regression analysis were used to analyze the results.
Demographic data showed significant differences between patients and controls in smoking (P < 0.01), body mass index (P < 0.05), family history of atherosclerotic disease (P < 0.01), and education (P < 0.01). Significant differences were also observed in oral health data, in the detection of P. gingivalis (P < 0.001), P. intermedia (P < 0.01), P. nigrescens (P < 0.001), and T. forsythia (P < 0.001) and in the levels of MMP-8 and MMP-9 in GCF between patients and controls. T. forsythia[odds ratio(OR) 10.1; P = 0.001] and age (OR 5.54; P = 0.008) appeared to be the main independent predictors for high MMP-8 in GCF. Patients had significantly higher total cholesterol (P < 0.01), low-density lipoprotein cholesterol (P = 0.05), and triglycerides (P < = 0.01) than controls. Plasma levels of MMP-9 were significantly higher in patients than in controls (P = 0.001).
Specific periodontal microorganisms appeared to induce host response, with increased release of MMP-8 and MMP-9 in gingival pockets as well as of MMP-9 in plasma, possibly triggering its up-regulation in blood.
研究牙龈沟液（GCF）中基质金属蛋白酶（MMP）-8和-9水平以及牙周病原体同时存在的情况，同时研究血液中MMP-9和胆固醇水平。虽然细菌病原体是引发牙周疾病过程所必需的，但在一些个体中，对细菌的反应可能导致宿主过度反应，从而引发全身性炎症反应。
分析了33名有16年牙周疾病病史和口腔健康记录的受试者以及31名牙周健康对照者的血液样本中的MMP-9和脂质。获取了有关教育程度、体重指数和动脉粥样硬化家族史的信息。采集GCF以测定MMP-8和MMP-9水平，并同时收集细菌样本用于聚合酶链反应评估伴放线放线杆菌、牙龈卟啉单胞菌、中间普氏菌、变黑普氏菌、福赛坦纳菌和具核梭杆菌。采用方差分析、卡方检验和多元逻辑回归分析来分析结果。
人口统计学数据显示，患者与对照组在吸烟（P < 0.01）、体重指数（P < 0.05）、动脉粥样硬化疾病家族史（P < 0.01）和教育程度（P < 0.01）方面存在显著差异。在口腔健康数据方面，患者与对照组在牙龈卟啉单胞菌（P < 0.001）、中间普氏菌（P < 0.01）、变黑普氏菌（P < 0.001）和福赛坦纳菌（P < 0.001）的检测以及GCF中MMP-8和MMP-9水平方面也存在显著差异。福赛坦纳菌[比值比（OR）10.1；P = 0.001]和年龄（OR 5.54；P = 0.008）似乎是GCF中高MMP-8的主要独立预测因素。患者的总胆固醇（P < 0.01）、低密度脂蛋白胆固醇（P = 0.05）和甘油三酯（P <= 0.01）显著高于对照组。患者血浆中MMP-9水平显著高于对照组（P = 0.001）。
特定的牙周微生物似乎会诱导宿主反应，导致牙龈袋中MMP-8和MMP-9以及血浆中MMP-9的释放增加，可能会引发其在血液中的上调。
