Taketa K, Ichikawa E, Yamamoto T, Kato H, Matsuura S, Taga H, Hirai H
Health Research Center, Kagawa University, Takamatsu, Japan.
Tumour Biol. 1990;11(4):220-8.
By means of Datura stramonium agglutinin (DSA) affinity electrophoresis, human alpha-fetoprotein (AFP) was resolved into five bands, AFP-D1, D2, D3, D4 and D5, in order of decreasing mobility. AFP-D1, which had no affinity for DSA, comprised more than 84% of the intensity of total AFP bands. The percentage of AFP-D2 increased marginally in hepatitis and liver cirrhosis with or without hepatocellular carcinoma. AFP-D3 increased characteristically in hepatocellular carcinoma and AFP-D4, which had the highest affinity for DSA, increased up to 12% in other tumors, mostly of gastrointestinal origin. AFP-D5 showed no consistent changes among the benign and malignant diseases. The assay of AFP-D3 and D4 proved useful as a highly specific marker of hepatocellular carcinoma and other tumors, respectively.
借助曼陀罗凝集素（DSA）亲和电泳，人甲胎蛋白（AFP）可分离为五条带，即AFP-D1、D2、D3、D4和D5，迁移率依次降低。对DSA无亲和力的AFP-D1，占总AFP条带强度的84%以上。在伴有或不伴有肝细胞癌的肝炎和肝硬化中，AFP-D2的百分比略有增加。AFP-D3在肝细胞癌中特征性增加，而对DSA亲和力最高的AFP-D4在其他肿瘤（大多起源于胃肠道）中增加至12%。AFP-D5在良性和恶性疾病中未显示出一致变化。结果表明，AFP-D3和AFP-D4检测分别作为肝细胞癌和其他肿瘤的高特异性标志物很有用。
