Department of Medical Chemistry, Medical University of Białystok, Poland.
Clin Exp Med. 2011 Jun;11(2):81-8. doi: 10.1007/s10238-010-0113-6. Epub 2010 Oct 17.
It is suggested that gastric mucins, and in particular some specific glycan structures that can act as carbohydrate receptors, are involved in the interactions with Helicobacter pylori adhesins. The main aim of our study was to evaluate glycosylation pattern of glycoproteins of gastric juice before and at the end of eradication therapy. Gastric juices were taken from 13 clinical patients and subjected to analysis. Pooled fractions of the void volume obtained after gel filtration were subjected to ELISA tests. To assess the relative amounts of carbohydrate structures, lectins and monoclonal antibodies were used. Changes in the level of MUC 1 and MUC 5AC mucins and of carbohydrate structures, which are suggested to be receptors for Helicobacter pylori adhesins, were observed by the end of the eradication treatment. Our results support the idea about the involvement of MUC 5AC and MUC 1 with some specific sugar structures in the mechanism of Helicobacter pylori infection.
据认为，胃粘蛋白，特别是一些可以作为碳水化合物受体的特定糖链结构，参与了与幽门螺杆菌黏附素的相互作用。我们研究的主要目的是评估根除治疗前后胃液中糖蛋白的糖基化模式。从 13 名临床患者中采集胃液并进行分析。凝胶过滤后获得的空隙体积的合并级分进行 ELISA 测试。为了评估碳水化合物结构的相对含量，使用了凝集素和单克隆抗体。通过根除治疗结束时观察到 MUC1 和 MUC5AC 粘蛋白以及被认为是幽门螺杆菌黏附素受体的碳水化合物结构水平的变化。我们的结果支持 MUC5AC 和 MUC1 与幽门螺杆菌感染机制中某些特定糖结构的参与的观点。
