Vijverberg K, Schreuder G M, Kenter M J, Naipal A M, van Rood J J, Giphart M J
Department of Immunohematology and Blood Bank, University Hospital, Leiden, The Netherlands.
Tissue Antigens. 1990 Apr;35(4):165-71. doi: 10.1111/j.1399-0039.1990.tb01774.x.
Our group recently developed oligonucleotide probes associated to the TA10 and 2B3 specificities (1). Unambiguous typings were observed in a panel of homozygous typing cells and a family, using 32P-end labeled probes and PCR-amplified DNA (DQB exon 2). To investigate whether these TA10 and 2B3 associated oligonucleotides could be used in routine HLA-typing we extended the study to a panel of healthy, unrelated individuals. When TA10 and 2B3 typings by oligonucleotides were compared with those obtained in routine serology a complete correlation was observed for TA10. A discrepancy was seen for 2B3 typing in material obtained from DQw5- (and possibly DQw4)-positive individuals which could be explained by the CYNAP (cytotoxicity-negative, absorption-positive) phenomenon, using the IIB3 monoclonal antibody in routine tissue typing. Our results suggest that HLA-DQB oligonucleotide typing for TA10 and 2B3 is an accurate and reliable method and can be used effectively in routine HLA typing.
我们小组最近开发了与TA10和2B3特异性相关的寡核苷酸探针(1)。使用32P末端标记探针和PCR扩增的DNA(DQB第2外显子)，在一组纯合分型细胞和一个家系中观察到明确的分型。为了研究这些与TA10和2B3相关的寡核苷酸是否可用于常规HLA分型，我们将研究扩展到一组健康的、无亲缘关系的个体。当将通过寡核苷酸进行的TA10和2B3分型与常规血清学获得的分型进行比较时，观察到TA10完全相关。在从DQw5-(可能还有DQw4)-阳性个体获得的材料中，2B3分型出现了差异，这可以用在常规组织分型中使用IIB3单克隆抗体的CYNAP(细胞毒性阴性、吸收阳性)现象来解释。我们的结果表明，针对TA10和2B3的HLA-DQB寡核苷酸分型是一种准确可靠的方法，可有效地用于常规HLA分型。
