Kenter M J, Anholts J D, Schreuder G M, van Eggermond M C, Ghyselen G M, van Rood J J, Giphart M J
Department of Immunohaematology, University Hospital, Leiden, The Netherlands.
Hum Immunol. 1989 Jan;24(1):65-73. doi: 10.1016/0198-8859(89)90047-5.
Oligonucleotide probes specific for the serologically defined TA10 and 2B3 specificities were selected based on a comparison of the available HLA-DQ beta sequences. Panel and family segregation studies confirm a complete correlation between the reactivities of the selected probes and the TA10/IIB3 antibodies. The Glu residue at position 45 of the HLA-DQ beta chain is specific for the TA10 determinants, and a DQ beta Gly-Val-Tyr sequence is found at position 45-47 for all 2B3-positive DQ beta chains.
基于对现有HLA - DQβ序列的比较，选择了针对血清学定义的TA10和2B3特异性的寡核苷酸探针。群体和家系分离研究证实，所选探针的反应性与TA10/IIB3抗体之间存在完全相关性。HLA - DQβ链第45位的Glu残基对TA10决定簇具有特异性，并且在所有2B3阳性的DQβ链的第45 - 47位发现了DQβ Gly - Val - Tyr序列。
