Inoue Yusuke, Izawa Kiyoko, Tojo Arinobu, Sekine Rieko, Okubo Toshiyuki, Ohtomo Kuni
Department of Radiology, Institute of Medical Science, University of Tokyo, Japan.
Mol Imaging. 2006 Apr-Jun;5(2):53-6.
The identification of organs bearing luciferase activity by in vivo bioluminescence imaging (BLI) is often difficult, and ex vivo imaging of excised organs plays a complementary role. This study investigated the importance of exposure to the atmosphere in ex vivo BLI. Mice were inoculated with murine pro-B cell line Ba/F3 transduced with firefly luciferase and p190 BCR-ABL. They were killed following in vivo BLI, and whole-body imaging was done after death and then after intraperitoneal air injection. In addition, the right knee was exposed and imaged before and after the adjacent bones were cut. Extensive light signals were seen on in vivo imaging. The luminescence disappeared after the animal was killed, and air injection restored the light emission from the abdomen only, suggesting a critical role of atmospheric oxygen in luminescence after death. Although no substantial light signal at the right knee was seen before bone cutting, light emission was evident after cutting. In conclusion, in ex vivo BLI, light emission requires exposure to the atmosphere. Bone destruction is required to demonstrate luciferase activity in the bone marrow after death.
通过体内生物发光成像（BLI）识别具有荧光素酶活性的器官通常很困难，而切除器官的离体成像则起到补充作用。本研究调查了离体BLI中暴露于大气的重要性。用萤火虫荧光素酶和p190 BCR-ABL转导的小鼠前B细胞系Ba/F3接种小鼠。在体内BLI后将它们处死，死后进行全身成像，然后在腹腔注射空气后再次成像。此外，暴露右膝并在切割相邻骨骼前后进行成像。在体内成像时可见广泛的光信号。动物死后发光消失，空气注射仅恢复了腹部的发光，表明大气中的氧气在死后发光中起关键作用。虽然在切割骨骼前右膝未见到大量光信号，但切割后发光明显。总之，在离体BLI中，发光需要暴露于大气。死后需要破坏骨骼以证明骨髓中的荧光素酶活性。
