Zacharakis Giannis, Shih Helen, Ripoll Jorge, Weissleder Ralph, Ntziachristos Vasilis
Harvard Medical School, MA, USA.
Mol Imaging. 2006 Jul;5(3):153-9.
We investigated the capacity of normalized transillumination methods in imaging fluorescent proteins in visible light, in phantoms and in live mice. We demonstrate significant imaging improvements over epi-illumination imaging, as commonly applied today. Of particular importance is the significant betterment in contrast achieved, due to minimization of the influence of autofluorescence and the enhancement of depth sensitivity compared to epi-illumination imaging. Although normalized transillumination does not match the accuracy and quantification capacity of tomographic imaging, it nevertheless attains key advantages over other planar optical imaging methods while preserving implementation simplicity. Due to the balance achieved between simplicity and accuracy, normalized transillumination approaches could serve as an important alternative molecular imaging method.
我们研究了归一化透照方法在可见光下对荧光蛋白进行成像的能力，包括在模型和活体小鼠中的成像。我们证明，与目前常用的落射照明成像相比，该方法在成像方面有显著改进。特别重要的是，由于与落射照明成像相比，自发荧光的影响最小化且深度敏感性增强，从而实现了对比度的显著提高。尽管归一化透照在准确性和定量能力方面无法与断层成像相匹配，但它在保持实施简单性的同时，相对于其他平面光学成像方法仍具有关键优势。由于在简单性和准确性之间取得了平衡，归一化透照方法可作为一种重要的替代分子成像方法。
