Bruns M, Kratzberg T, Zeller W, Lehmann-Grube F
Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Federal Republic of Germany.
Virology. 1990 Aug;177(2):615-24. doi: 10.1016/0042-6822(90)90527-x.
During persistent infection of mouse L cells with strain Armstrong lymphocytic choriomeningitis virus, the latter undergoes characteristic changes, including loss of mouse pathogenicity and failure to form plaques on cultivated cells. We call this virus L(Arm) and have analyzed transcription and translation of its S-RNA, which codes for the viral nucleoprotein (NP) and the glycoprotein precursor (GP-C). In L(Arm) virus-infected L cells, S-RNA and genomic-sized viral complementary S-RNA (VC-S-RNA) were detected and, in addition, considerable quantities of shortened molecules of either species. The cells' content of NP was high, but they contained little GP-C; instead, a viral glycoprotein with MW 65,000 was present. We propose a hypothesis in which it is assumed that along the VC-S-RNA there is more than one recognition site for the viral RNA-dependent RNA polymerase, which leads to the generation of truncated forms of S-RNA, VC-S-RNA, and mRNA for GP-C; this, in turn, results in relative overproduction of NP and relative underproduction of GP-C as well as the emergence of a new form of viral glycoprotein.
在用阿姆斯特朗株淋巴细胞性脉络丛脑膜炎病毒持续感染小鼠L细胞的过程中，该病毒会发生特征性变化，包括失去对小鼠的致病性以及无法在培养细胞上形成蚀斑。我们将这种病毒称为L(Arm)，并分析了其编码病毒核蛋白（NP）和糖蛋白前体（GP-C）的S-RNA的转录和翻译情况。在感染了L(Arm)病毒的L细胞中，检测到了S-RNA和基因组大小的病毒互补S-RNA（VC-S-RNA），此外，还发现了大量这两种类型的缩短分子。细胞中NP的含量很高，但GP-C的含量很少；相反，存在一种分子量为65,000的病毒糖蛋白。我们提出一种假说，假定沿着VC-S-RNA存在不止一个病毒RNA依赖性RNA聚合酶的识别位点，这导致产生S-RNA、VC-S-RNA和GP-C的mRNA的截短形式；这反过来又导致NP相对过量产生、GP-C相对产量不足以及一种新形式的病毒糖蛋白出现。
