Fuller-Pace F V, Southern P J
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Virology. 1988 Jan;162(1):260-3. doi: 10.1016/0042-6822(88)90419-9.
We have analyzed the accumulation of viral genomic and messenger RNAs in tissue culture cells during the first 24 hr of acute infection with lymphocytic choriomeningitis virus (LCMV). This has allowed comparison of the relative amounts of the genomic L and S RNAs (both genomic sense and genomic complementary sense) and of nucleoprotein (NP) and glycoprotein precursor (GP-C) mRNAs. Using these techniques NP mRNA was detected simultaneously with genomic S RNA, but the amount of NP mRNA accumulating during this period of infection was higher than that of GP-C mRNA. This is consistent with a model for ambisense RNA transcription and replication proposed by D.D. Auperin, V. Romanowski, M. Galinski and D. H. L. Bishop (J. Virol. 52: 897-904, 1984). The accumulation of S RNA exceeded that of L RNA and, for both L and S RNAs, the amount of genomic sense RNA was higher than that of genomic complementary RNA.
我们分析了淋巴细胞性脉络丛脑膜炎病毒（LCMV）急性感染最初24小时期间病毒基因组RNA和信使RNA在组织培养细胞中的积累情况。这使得我们能够比较基因组L和S RNA（基因组意义链和基因组互补意义链）以及核蛋白（NP）和糖蛋白前体（GP-C）mRNA的相对含量。使用这些技术，在检测到基因组S RNA的同时也检测到了NP mRNA，但在感染这段时间内积累的NP mRNA量高于GP-C mRNA。这与D.D.奥珀林、V.罗曼诺夫斯基、M.加林斯基和D.H.L.毕晓普提出的双义RNA转录和复制模型（《病毒学杂志》52: 897 - 904, 1984）一致。S RNA的积累超过了L RNA，并且对于L和S RNA而言，基因组意义链RNA的量高于基因组互补RNA。
