Romanowski V, Matsuura Y, Bishop D H
Virus Res. 1985 Sep;3(2):101-14. doi: 10.1016/0168-1702(85)90001-2.
Previous studies have reported that the 3' half of the small, S, RNA species of the WE strain of lymphocytic choriomeningitis (LCM) virus codes for the viral nucleoprotein in a subgenomic, viral-complementary, mRNA species (Romanowski, V. and Bishop, D.H.L. (1985) Virus Res. 2, 35-51). The complete sequence of the LCM-WE S RNA has now been obtained, indicating that the 5' half of the RNA codes for the viral glycoprotein precursor in a viral-sense sequence that does not overlap the N gene. It is concluded that, like Pichinde virus (Auperin, D. et al. (1984) J. Virol. 52, 897-904), LCM has an ambisense S RNA coding strategy. The LCM-WE S RNA is 3375 nucleotides in length, has a size of 1.14 X 10(6) Da and base composition of 26.1% A, 23.2% C, 21.5% G, 29.2% U. The 3' and 5' end sequences of the S RNA are complementary for some 30 nucleotides, depending on the arrangement. The non-coding regions at the two ends are 77 (5') and 60 (3') nucleotides long. The glycoprotein precursor has a primary amino acid size of 56293 Da and is rich in potential glycosylation sites as well as histidine and cysteine residues. It has both amino and carboxy proximal hydrophobic regions. The LCM-WE S RNA and predicted protein sequence data have been compared to those of Pichinde arena-virus. Extensive RNA and protein sequence homology exists for the two S RNA species, although the homology for the glycoprotein sequences of the two viruses (39%) is less than the 50% observed for the two viral nucleoproteins.
先前的研究报道，淋巴细胞性脉络丛脑膜炎（LCM）病毒WE株的小S RNA种类的3' 端一半在一个亚基因组、病毒互补的mRNA种类中编码病毒核蛋白（Romanowski, V.和Bishop, D.H.L.（1985年）《病毒研究》2, 35 - 51）。现在已经获得了LCM - WE S RNA的完整序列，表明该RNA的5' 端一半在一个不与N基因重叠的病毒正义序列中编码病毒糖蛋白前体。得出的结论是，与皮钦德病毒（Auperin, D.等人（1984年）《病毒学杂志》52, 897 - 904）一样，LCM具有一种双义S RNA编码策略。LCM - WE S RNA长度为3375个核苷酸，大小为1.14×10(6) Da，碱基组成分别为26.1% A、23.2% C、21.5% G、29.2% U。S RNA的3' 和5' 端序列在约30个核苷酸处互补，具体取决于排列方式。两端的非编码区长度分别为77（5'）和60（3'）个核苷酸。糖蛋白前体的一级氨基酸大小为56293 Da，富含潜在的糖基化位点以及组氨酸和半胱氨酸残基。它既有氨基近端又有羧基近端疏水区域。已将LCM - WE S RNA和预测的蛋白质序列数据与皮钦德沙粒病毒的进行比较.两种S RNA种类存在广泛的RNA和蛋白质序列同源性，尽管两种病毒糖蛋白序列的同源性（39%）低于两种病毒核蛋白所观察到的50%。
