Schulze-Osthoff K, Risau W, Vollmer E, Sorg C
Institute of Experimental Dermatology, University of Münster, West Germany.
Am J Pathol. 1990 Jul;137(1):85-92.
Basic fibroblast growth factor (bFGF) is thought to be of major importance for fibrosis and angiogenesis. Despite intensive studies dealing with the biochemistry and multiple biologic effects of bFGF, the cellular distribution is virtually unknown. Therefore, using the indirect immunoperoxidase technique, we examined the effect of bFGF on a large pattern of normal, inflammatory, and tumorous human tissues. Staining was performed on cryostat sections with a highly specific affinity-purified antiserum. In normal tissues, especially those of the thymus and placenta, mainly dendritic cells contained the growth factor. High levels of bFGF were also detected in basal cells and gland epithelial cells of skin biopsies. A conspicuous expression was observed in chronic inflammatory tissues corresponding to a generally pronounced proliferation of fibroblasts and endothelial cells in these situations. Tumors revealed a very heterogenous staining pattern. In some lesions, bFGF was predominantly present in infiltrating and endothelial cells. In several, neoplasms tumor cells exhibited an intensive staining. In some, especially vascular tumors, bFGF could not be detected. From the staining results it is concluded that angiogenesis is not simply controlled by the presence of bFGF but is mediated by a balance of several angiogenic inducers and inhibitors.
碱性成纤维细胞生长因子（bFGF）被认为在纤维化和血管生成中起主要作用。尽管对bFGF的生物化学和多种生物学效应进行了深入研究，但其细胞分布实际上仍不清楚。因此，我们使用间接免疫过氧化物酶技术，研究了bFGF对大量正常、炎症和肿瘤性人体组织的影响。用高度特异性的亲和纯化抗血清对冷冻切片进行染色。在正常组织中，尤其是胸腺和胎盘组织，主要是树突状细胞含有这种生长因子。在皮肤活检的基底细胞和腺上皮细胞中也检测到高水平的bFGF。在慢性炎症组织中观察到明显的表达，这与这些情况下成纤维细胞和内皮细胞普遍明显的增殖相对应。肿瘤显示出非常异质的染色模式。在一些病变中，bFGF主要存在于浸润细胞和内皮细胞中。在一些肿瘤中，肿瘤细胞呈现强烈染色。在一些肿瘤中，尤其是血管肿瘤，未检测到bFGF。从染色结果可以得出结论，血管生成不仅仅由bFGF的存在控制，而是由多种血管生成诱导剂和抑制剂的平衡介导。
