Seyama T, Ko J H, Ohe M, Sasaoka N, Okada A, Gomi H, Yoneda A, Ueda J, Nishibori M, Okamoto S, Maeda Y, Watanabe T
Graduate School of Agriculture, Hokkaido University, Sapporo, Japan.
Biochem Genet. 2006 Oct;44(9-10):437-48. doi: 10.1007/s10528-006-9040-3. Epub 2006 Sep 6.
A single amino acid substitution between Asn and Ser at position 631 in the chicken Mx protein has been reported to determine resistant and sensitive antiviral activity. In this study, we investigate whether various kinds of chicken breeds and jungle fowls carry the resistant or sensitive Mx allelic gene by using the mismatched PCR-restriction fragment length polymorphism (RFLP) technique. In total, 271 samples from 36 strains of 17 chicken breeds and from 3 kinds of jungle fowls were examined. The rates of the resistant Mx gene and sensitive gene were 59.2% and 40.8%, respectively. Only a Red jungle fowl captured in Laos carried the resistant Mx gene, and the other three Red jungle fowls from Indonesia and Gray and Green jungle fowls all had the sensitive Mx gene. These results were confirmed by the determination of amino acid sequences in the GTPase effector domain of jungle fowls.
据报道，鸡Mx蛋白631位的天冬酰胺（Asn）和丝氨酸（Ser）之间的单个氨基酸取代决定了抗病毒活性的抗性和敏感性。在本研究中，我们使用错配PCR-限制性片段长度多态性（RFLP）技术，调查各种鸡品种和原鸡是否携带抗性或敏感性Mx等位基因。总共检测了来自17个鸡品种的36个品系以及3种原鸡的271个样本。抗性Mx基因和敏感性基因的比例分别为59.2%和40.8%。仅一只在老挝捕获的红原鸡携带抗性Mx基因，而另外三只来自印度尼西亚的红原鸡以及灰原鸡和绿原鸡均具有敏感性Mx基因。这些结果通过对原鸡GTPase效应结构域氨基酸序列的测定得到了证实。
