抗体结合是1型人类免疫缺陷病毒中和效率的主要决定因素。
Antibody binding is a dominant determinant of the efficiency of human immunodeficiency virus type 1 neutralization.
作者信息
Yang Xinzhen, Lipchina Inna, Cocklin Simon, Chaiken Irwin, Sodroski Joseph
机构信息
Beth Israel Deaconess Medical Center, 330 Brookline Avenue, R.E. 213A, Boston, MA 02215, USA.
出版信息
J Virol. 2006 Nov;80(22):11404-8. doi: 10.1128/JVI.01102-06. Epub 2006 Sep 6.
Abstract
Primary and laboratory-adapted variants of human immunodeficiency virus type 1 (HIV-1) exhibit a wide range of sensitivities to neutralization by antibodies directed against the viral envelope glycoproteins. An antibody directed against an artificial FLAG epitope inserted into the envelope glycoproteins of three HIV-1 isolates with vastly different neutralization sensitivities inhibited all three viruses equivalently. Thus, naturally occurring HIV-1 isolates that are neutralization resistant are not necessarily more impervious to the inhibitory consequences of bound antibody. Moreover, the binding affinity of the anti-FLAG antibody correlated with neutralizing potency, underscoring the dominant impact on neutralization of antibody binding to the envelope glycoproteins.
摘要
1型人类免疫缺陷病毒（HIV-1）的原始毒株和实验室适应性变体对针对病毒包膜糖蛋白的抗体中和作用表现出广泛的敏感性。一种针对插入到三种具有截然不同中和敏感性的HIV-1分离株包膜糖蛋白中的人工FLAG表位的抗体，能同等程度地抑制这三种病毒。因此，具有中和抗性的天然HIV-1分离株不一定对结合抗体的抑制作用更具抗性。此外，抗FLAG抗体的结合亲和力与中和效力相关，突出了抗体与包膜糖蛋白结合对中和作用的主要影响。
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本文引用的文献
1
Neutralizing antibody responses drive the evolution of human immunodeficiency virus type 1 envelope during recent HIV infection.在近期的HIV感染过程中，中和抗体反应推动了1型人类免疫缺陷病毒包膜的演变。
Proc Natl Acad Sci U S A. 2005 Dec 20;102(51):18514-9. doi: 10.1073/pnas.0504658102. Epub 2005 Dec 9.
2
HIV-1: the confounding variables of virus neutralization.人类免疫缺陷病毒1型：病毒中和的混杂变量
Curr Drug Targets Infect Disord. 2005 Jun;5(2):157-70. doi: 10.2174/1568005054201535.
3
An unrelated monoclonal antibody neutralizes human immunodeficiency virus type 1 by binding to an artificial epitope engineered in a functionally neutral region of the viral envelope glycoproteins.一种无关的单克隆抗体通过与在病毒包膜糖蛋白功能中性区域设计的人工表位结合来中和1型人类免疫缺陷病毒。
J Virol. 2005 May;79(9):5616-24. doi: 10.1128/JVI.79.9.5616-5624.2005.
4
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J Virol. 2005 Mar;79(6):3500-8. doi: 10.1128/JVI.79.6.3500-3508.2005.
5
Modulation of Env content in virions of simian immunodeficiency virus: correlation with cell surface expression and virion infectivity.猴免疫缺陷病毒病毒粒子中Env含量的调节：与细胞表面表达及病毒粒子感染性的相关性
J Virol. 2004 Jul;78(13):6775-85. doi: 10.1128/JVI.78.13.6775-6785.2004.
6
Native HIV type 1 virion surface structures: relationships between antibody binding and neutralization or lessons from the viral capture assay.天然1型人类免疫缺陷病毒病毒体表面结构：抗体结合与中和作用之间的关系或病毒捕获试验的经验教训
AIDS Res Hum Retroviruses. 2004 Apr;20(4):435-41. doi: 10.1089/088922204323048186.
7
HIV sequence databases.HIV序列数据库。
AIDS Rev. 2003 Jan-Mar;5(1):52-61.
8
Multiple interactions across the surface of the gp120 core structure determine the global neutralization resistance phenotype of human immunodeficiency virus type 1.gp120核心结构表面的多种相互作用决定了1型人类免疫缺陷病毒的整体中和抗性表型。
J Virol. 2003 Jul;77(14):8061-71. doi: 10.1128/jvi.77.14.8061-8071.2003.
9
Antibody neutralization and escape by HIV-1.HIV-1的抗体中和作用与逃逸
Nature. 2003 Mar 20;422(6929):307-12. doi: 10.1038/nature01470.
10
Rapid evolution of the neutralizing antibody response to HIV type 1 infection.对1型人类免疫缺陷病毒感染的中和抗体反应的快速演变。
Proc Natl Acad Sci U S A. 2003 Apr 1;100(7):4144-9. doi: 10.1073/pnas.0630530100. Epub 2003 Mar 18.
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