• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

牙龈卟啉单胞菌赖氨酸2,3-氨基变位酶的酶促激活

Enzymatic activation of lysine 2,3-aminomutase from Porphyromonas gingivalis.

作者信息

Brazeau Brian J, Gort Steven J, Jessen Holly J, Andrew Amy J, Liao Hans H

机构信息

Biotechnology Development Center-Eddyville, Cargill Incorporated, 1 Cargill Drive, Eddyville, IA 52553, USA.

出版信息

Appl Environ Microbiol. 2006 Sep;72(9):6402-4. doi: 10.1128/AEM.01143-06.

DOI:10.1128/AEM.01143-06
PMID:16957271
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1563663/
Abstract

The development of lysine 2,3-aminomutase as a robust biocatalyst hinges on the development of an in vivo activation system to trigger catalysis. This is the first report to show that, in the absence of chemical reductants, lysine 2,3-aminomutase activity is dependent upon the presence of flavodoxin, ferredoxin, or flavodoxin-NADP(+) reductase.

摘要

赖氨酸2,3-氨基变位酶作为一种强大的生物催化剂的发展取决于体内激活系统的发展以触发催化作用。这是第一份表明在没有化学还原剂的情况下，赖氨酸2,3-氨基变位酶活性依赖于黄素氧还蛋白、铁氧化还原蛋白或黄素氧还蛋白-NADP(+)还原酶存在的报告。

相似文献

1
Enzymatic activation of lysine 2,3-aminomutase from Porphyromonas gingivalis.牙龈卟啉单胞菌赖氨酸2,3-氨基变位酶的酶促激活
Appl Environ Microbiol. 2006 Sep;72(9):6402-4. doi: 10.1128/AEM.01143-06.
2
Routes of flavodoxin and ferredoxin reduction in Escherichia coli. CoA-acylating pyruvate: flavodoxin and NADPH: flavodoxin oxidoreductases participating in the activation of pyruvate formate-lyase.大肠杆菌中黄素氧还蛋白和铁氧还蛋白的还原途径。参与丙酮酸甲酸裂解酶激活的辅酶A酰化丙酮酸：黄素氧还蛋白和NADPH：黄素氧还蛋白氧化还原酶。
Eur J Biochem. 1982 Apr;123(3):563-9.
3
Recombinant human cytochrome P450 1A2 and an N-terminal-truncated form: construction, purification, aggregation properties, and interactions with flavodoxin, ferredoxin, and NADPH-cytochrome P450 reductase.重组人细胞色素P450 1A2及其N端截短形式：构建、纯化、聚集特性以及与黄素氧还蛋白、铁氧还蛋白和NADPH-细胞色素P450还原酶的相互作用
Arch Biochem Biophys. 1996 Mar 1;327(1):11-9. doi: 10.1006/abbi.1996.0086.
4
Lys75 of Anabaena ferredoxin-NADP+ reductase is a critical residue for binding ferredoxin and flavodoxin during electron transfer.鱼腥藻铁氧化还原蛋白-NADP+还原酶的赖氨酸75是电子转移过程中结合铁氧化还原蛋白和黄素氧化还原蛋白的关键残基。
Biochemistry. 1998 Sep 29;37(39):13604-13. doi: 10.1021/bi9807411.
5
Kinetic and biochemical analysis of the mechanism of action of lysine 5,6-aminomutase.赖氨酸5,6-氨基变位酶作用机制的动力学与生化分析
Arch Biochem Biophys. 2003 Oct 1;418(1):49-54. doi: 10.1016/s0003-9861(03)00346-1.
6
Kinetic, spectroscopic and thermodynamic characterization of the Mycobacterium tuberculosis adrenodoxin reductase homologue FprA.结核分枝杆菌肾上腺皮质铁氧化还原蛋白还原酶同源物FprA的动力学、光谱学和热力学特性
Biochem J. 2003 Jun 1;372(Pt 2):317-27. doi: 10.1042/BJ20021692.
7
Cyanobacterial flavodoxin complements ferredoxin deficiency in knocked-down transgenic tobacco plants.蓝藻黄素氧化还原蛋白可弥补敲除型转基因烟草植株中ferredoxin 的缺乏。
Plant J. 2011 Mar;65(6):922-35. doi: 10.1111/j.1365-313X.2010.04479.x. Epub 2011 Feb 21.
8
Flavodoxin is required for the activation of the anaerobic ribonucleotide reductase.黄素氧还蛋白是厌氧核糖核苷酸还原酶激活所必需的。
Biochem Biophys Res Commun. 1993 Dec 15;197(2):792-7. doi: 10.1006/bbrc.1993.2548.
9
Ferredoxin-NADP(+) reductase uses the same site for the interaction with ferredoxin and flavodoxin.铁氧化还原蛋白-NADP(+)还原酶与铁氧化还原蛋白和黄素氧化还原蛋白相互作用时使用相同的位点。
J Biol Inorg Chem. 1999 Oct;4(5):568-78.
10
Intramolecular and intracomplex electron transfer in redox proteins.
Prog Clin Biol Res. 1988;274:401-18.

引用本文的文献

1
A heme-dependent enzyme forms the hydrazine in the antibiotic negamycin.一种血红素依赖性酶在抗生素Negamycin中形成肼。
Nat Chem Biol. 2025 May 1. doi: 10.1038/s41589-025-01898-0.
2
Structural Basis for Allostery in PLP-dependent Enzymes.磷酸吡哆醛依赖性酶变构调节的结构基础。
Front Mol Biosci. 2022 Apr 25;9:884281. doi: 10.3389/fmolb.2022.884281. eCollection 2022.
3
Crystal structure of AdoMet radical enzyme 7-carboxy-7-deazaguanine synthase from Escherichia coli suggests how modifications near [4Fe-4S] cluster engender flavodoxin specificity.大肠杆菌 AdoMet 自由基酶 7-羧基-7-脱氮鸟嘌呤合酶的晶体结构揭示了[4Fe-4S]簇附近的修饰如何产生黄素蛋白特异性。
Protein Sci. 2019 Jan;28(1):202-215. doi: 10.1002/pro.3529.
4
Structural Insights into Thioether Bond Formation in the Biosynthesis of Sactipeptides.硫醚键在 sactipeptides 生物合成中的结构研究
J Am Chem Soc. 2017 Aug 30;139(34):11734-11744. doi: 10.1021/jacs.7b01283. Epub 2017 Aug 21.
5
Biochemical and Spectroscopic Characterization of a Radical S-Adenosyl-L-methionine Enzyme Involved in the Formation of a Peptide Thioether Cross-Link.参与肽硫醚交联形成的自由基S-腺苷-L-甲硫氨酸酶的生化与光谱表征
Biochemistry. 2016 Apr 12;55(14):2122-34. doi: 10.1021/acs.biochem.6b00145. Epub 2016 Apr 1.
6
Chemical and Biological Reduction of the Radical SAM Enzyme 7-Carboxy-7-deazaguanine [corrected] Synthase.自由基SAM酶7-羧基-7-脱氮鸟嘌呤[校正后]合酶的化学和生物还原
Biochemistry. 2015 May 12;54(18):2903-10. doi: 10.1021/acs.biochem.5b00210. Epub 2015 May 1.
7
Dph3 is an electron donor for Dph1-Dph2 in the first step of eukaryotic diphthamide biosynthesis.Dph3 是真核生物二氢尿嘧啶生物合成第一步中 Dph1-Dph2 的电子供体。
J Am Chem Soc. 2014 Feb 5;136(5):1754-7. doi: 10.1021/ja4118957. Epub 2014 Jan 22.
8
Spectroscopic, steady-state kinetic, and mechanistic characterization of the radical SAM enzyme QueE, which catalyzes a complex cyclization reaction in the biosynthesis of 7-deazapurines.光谱学、稳态动力学和机制表征的 radical SAM 酶 QueE，它催化 7-脱氮嘌呤生物合成中的复杂环化反应。
Biochemistry. 2013 Jan 8;52(1):188-98. doi: 10.1021/bi301156w. Epub 2012 Dec 24.
9
Structural diversity in the AdoMet radical enzyme superfamily.S-腺苷甲硫氨酸自由基酶超家族中的结构多样性。
Biochim Biophys Acta. 2012 Nov;1824(11):1178-95. doi: 10.1016/j.bbapap.2012.04.006. Epub 2012 Apr 28.
10
Binding energy in the one-electron reductive cleavage of S-adenosylmethionine in lysine 2,3-aminomutase, a radical SAM enzyme.赖氨酸2,3-氨基变位酶（一种自由基S-腺苷甲硫氨酸酶）中S-腺苷甲硫氨酸单电子还原裂解的结合能。
Biochemistry. 2007 Nov 13;46(45):12889-95. doi: 10.1021/bi701745h. Epub 2007 Oct 18.

本文引用的文献

1
Trends and innovations in industrial biocatalysis for the production of fine chemicals.用于精细化学品生产的工业生物催化的趋势与创新
Curr Opin Biotechnol. 2004 Aug;15(4):272-9. doi: 10.1016/j.copbio.2004.06.011.
2
Dispelling the myths--biocatalysis in industrial synthesis.破除迷思——工业合成中的生物催化
Science. 2003 Mar 14;299(5613):1694-7. doi: 10.1126/science.1079237.
3
Radical mechanisms of enzymatic catalysis.酶催化的自由基机制。
Annu Rev Biochem. 2001;70:121-48. doi: 10.1146/annurev.biochem.70.1.121.
4
Industrial biocatalysis today and tomorrow.当今与未来的工业生物催化。
Nature. 2001 Jan 11;409(6817):258-68. doi: 10.1038/35051736.
5
Direct FeS cluster involvement in generation of a radical in lysine 2,3-aminomutase.直接参与赖氨酸2,3-氨基变位酶中自由基生成的硫化铁簇。
Biochemistry. 2000 Dec 26;39(51):15668-73. doi: 10.1021/bi0022184.
6
Biocatalysis for industrial production of fine chemicals.用于精细化学品工业生产的生物催化
Curr Opin Biotechnol. 1999 Dec;10(6):609-15. doi: 10.1016/s0958-1669(99)00042-7.
7
Escherichia coli biotin synthase: an investigation into the factors required for its activity and its sulfur donor.大肠杆菌生物素合成酶：对其活性所需因素及其硫供体的研究。
Arch Biochem Biophys. 1996 Feb 1;326(1):48-56. doi: 10.1006/abbi.1996.0045.
8
Escherichia coli ferredoxin NADP+ reductase: activation of E. coli anaerobic ribonucleotide reduction, cloning of the gene (fpr), and overexpression of the protein.大肠杆菌铁氧化还原蛋白NADP+还原酶：大肠杆菌厌氧核糖核苷酸还原的激活、基因（fpr）的克隆及该蛋白的过表达
J Bacteriol. 1993 Mar;175(6):1590-5. doi: 10.1128/jb.175.6.1590-1595.1993.
9
Biotin synthase from Escherichia coli, an investigation of the low molecular weight and protein components required for activity in vitro.来自大肠杆菌的生物素合成酶，对体外活性所需的低分子量和蛋白质成分的研究。
J Biol Chem. 1995 Aug 11;270(32):19158-65. doi: 10.1074/jbc.270.32.19158.
10
Single-step assembly of a gene and entire plasmid from large numbers of oligodeoxyribonucleotides.从大量寡脱氧核糖核苷酸一步组装基因和完整质粒。
Gene. 1995 Oct 16;164(1):49-53. doi: 10.1016/0378-1119(95)00511-4.