Yin Jieyun, Hou Xiaohua, Chen J D Z
Veterans Research and Education Foundation, VA Medical Center Transneuronix Inc, Oklahoma City, Oklahoma, USA.
Dig Dis Sci. 2006 Oct;51(10):1818-23. doi: 10.1007/s10620-006-9313-z.
The aims of this study were to investigate the role of interstitial cells of Cajal (ICCs) on small intestinal transit and its responses to exogenous pacing in W/W(v) mice. Eleven W/W(v) mice and their controls implanted with four pairs of gastrointestinal electrodes were used for testing the entrainment of slow waves. Another 20 W/W(v) mice and their controls equipped with a duodenal catheter and one pair of intestinal electrodes were used to test small intestinal transit represented by the geometric center (GC). Results were as follows. (1) The effect of pacing on slow wave frequency was sustained only in controls, and not in W/W(v) mice. (2) Both gastric and intestinal slow waves were completely entrained in controls and W/W(v) mice. Higher energy was required for pacing the stomach than the small intestine. (3) There was no significant difference in small intestinal transit between the controls and the W/W(v) mice (GC: 5.4 vs. 5.5). (4) Pacing showed no effects on small intestinal transit in either wild-type (GC: 5.4 vs. 5.6) or W/W(v) mice (GC: 5.5 vs. 5.7). We conclude that myenteric ICCs may not play an important role in the regulation of small intestinal transit in conscious mice. Gastric and intestinal pacing can be achieved without ICCs.
本研究旨在探讨Cajal间质细胞（ICCs）在W/W（v）小鼠小肠转运中的作用及其对外源性起搏的反应。11只W/W（v）小鼠及其植入四对胃肠电极的对照小鼠用于测试慢波的夹带情况。另外20只配备十二指肠导管和一对肠电极的W/W（v）小鼠及其对照小鼠用于测试以几何中心（GC）表示的小肠转运情况。结果如下：（1）起搏对慢波频率的影响仅在对照小鼠中持续存在，而在W/W（v）小鼠中则不然。（2）胃和肠的慢波在对照小鼠和W/W（v）小鼠中均完全被夹带。起搏胃所需的能量高于小肠。（3）对照小鼠和W/W（v）小鼠之间的小肠转运无显著差异（GC：5.4对5.5）。（4）起搏对野生型小鼠（GC：5.4对5.6）或W/W（v）小鼠（GC：5.5对5.7）的小肠转运均无影响。我们得出结论，肌间ICCs在清醒小鼠小肠转运的调节中可能不发挥重要作用。没有ICCs也可以实现胃和肠的起搏。
