Faculty of Chemical Engineering and Biotechnologies, National University of Science and Technology POLITEHNICA Bucharest, 1-7 Gheorghe Polizu St., 1st District, 011061 Bucharest, Romania.
National Institute for Research and Development in Microtechnologies-IMT Bucharest, 126A Erou Iancu Nicolae, 077190 Voluntari, Romania.
Mar Drugs. 2024 Sep 25;22(10):434. doi: 10.3390/md22100434.
Phycocyanin was extracted from using conventional extraction (CE), direct ultrasonic-assisted extraction (direct UAE), indirect ultrasonic-assisted extraction (indirect UAE), and microwave-assisted extraction (MAE) methods at different temperatures, extraction intervals, stirring rate, and power intensities while maintaining the same algae to solvent ratio (1:15 ). The optimization of the extraction parameters indicated that the direct UAE yielded the highest phycocyanin concentration (29.31 ± 0.33 mg/mL) and antioxidant activity (23.6 ± 0.56 mg TE/g algae), while MAE achieved the highest purity (Rp = 0.5 ± 0.002). Based on the R value, phycocyanin extract obtained by MAE (1:15 algae to solvent ratio, 40 min, 40 °C, and 900 rpm) was selected as active compound in an alginate-based hydrogel formulation designed as potential wound dressings. Phycocyanin extracts and loaded hydrogels were characterized by FT-IR analysis. SEM analysis confirmed a porous structure for both blank and phycocyanin loaded hydrogels, while the mechanical properties remained approximately unchanged in the presence of phycocyanin. Phycocyanin release kinetics was investigated at two pH values using Zero-order, First-order, Higuchi, and Korsmeyer-Peppas kinetics models. The Higuchi model best fitted the experimental results. The R value at higher pH was nearly 1, indicating a superior fit compared with lower pH values.
藻蓝蛋白是从 使用常规提取（CE）、直接超声辅助提取（direct UAE）、间接超声辅助提取（indirect UAE）和微波辅助提取（MAE）方法，在不同的温度、提取间隔、搅拌速度和功率强度下提取的，同时保持藻类与溶剂的比例相同（1:15）。提取参数的优化表明，直接 UAE 产生的藻蓝蛋白浓度最高（29.31 ± 0.33 mg/mL）和抗氧化活性（23.6 ± 0.56 mg TE/g 藻类），而 MAE 达到的纯度最高（Rp = 0.5 ± 0.002）。根据 R 值，MAE（1:15 藻类与溶剂的比例、40 分钟、40°C 和 900 rpm）获得的藻蓝蛋白提取物被选为藻酸盐基水凝胶配方中的活性化合物，该配方设计用作潜在的伤口敷料。藻蓝蛋白提取物和负载水凝胶通过 FT-IR 分析进行了表征。SEM 分析证实了空白和负载藻蓝蛋白的水凝胶均具有多孔结构，而在存在藻蓝蛋白的情况下，机械性能几乎保持不变。藻蓝蛋白释放动力学在两种 pH 值下使用零级、一级、Higuchi 和 Korsmeyer-Peppas 动力学模型进行了研究。Higuchi 模型最适合实验结果。在较高 pH 值下的 R 值接近 1，表明与较低 pH 值相比拟合效果更好。
