凝缩蛋白在有丝分裂核仁分离中的功能受核糖体DNA转录调控。
Condensin function in mitotic nucleolar segregation is regulated by rDNA transcription.
作者信息
Wang Bi-Dar, Butylin Pavel, Strunnikov Alexander
机构信息
NICHD, Laboratory of Gene Regulation and Development, Bethesda, Maryland 20892, USA.
出版信息
Cell Cycle. 2006 Oct;5(19):2260-7. doi: 10.4161/cc.5.19.3292. Epub 2006 Oct 1.
Abstract
Chromosome condensation is established and maintained by the condensin complex. The mechanisms governing loading of condensin onto specific chromosomal sites remain unknown. To elucidate the molecular pathways that determine condensin binding to the nucleolar organizer, a key condensin binding site, we analyzed the properties of condensin-bound sites within the rDNA repeat in budding yeast and demonstrated that the bulk of mitotic condensin binding to rDNA is reduced or eliminated when Pol I transcription is elevated. Conversely, when Pol I transcription is repressed either by rapamycin treatment or by promoter shut-off, condensin binding to rDNA is increased. This novel potential role for constitutive and/or periodic repression of Pol I transcription in rDNA condensin loading is an important factor in determining the segregation proficiency of NOR-containing chromosomes.
摘要
染色体凝聚由凝聚素复合体建立并维持。控制凝聚素加载到特定染色体位点的机制仍不清楚。为了阐明决定凝聚素与核仁组织区（一个关键的凝聚素结合位点）结合的分子途径，我们分析了芽殖酵母rDNA重复序列中凝聚素结合位点的特性，并证明当Pol I转录升高时，有丝分裂凝聚素与rDNA的大量结合会减少或消除。相反，当通过雷帕霉素处理或启动子关闭来抑制Pol I转录时，凝聚素与rDNA的结合会增加。Pol I转录的组成型和/或周期性抑制在rDNA凝聚素加载中的这一新潜在作用是决定含核仁组织区染色体分离能力的一个重要因素。
相似文献
1
Condensin function in mitotic nucleolar segregation is regulated by rDNA transcription.凝缩蛋白在有丝分裂核仁分离中的功能受核糖体DNA转录调控。
Cell Cycle. 2006 Oct;5(19):2260-7. doi: 10.4161/cc.5.19.3292. Epub 2006 Oct 1.
2
A case of selfish nucleolar segregation.一例自私的核仁分离现象。
Cell Cycle. 2005 Jan;4(1):113-7. doi: 10.4161/cc.4.1.1488. Epub 2005 Jan 21.
3
Transcriptional homogenization of rDNA repeats in the episome-based nucleolus induces genome-wide changes in the chromosomal distribution of condensin.基于附加体的核仁中rDNA重复序列的转录均质化诱导了凝缩蛋白在染色体分布上的全基因组变化。
Plasmid. 2008 Jan;59(1):45-53. doi: 10.1016/j.plasmid.2007.09.003. Epub 2007 Nov 19.
4
Cdc14p/FEAR pathway controls segregation of nucleolus in S. cerevisiae by facilitating condensin targeting to rDNA chromatin in anaphase.Cdc14p/FEAR途径通过促进后期凝聚素靶向rDNA染色质来控制酿酒酵母中核仁的分离。
Cell Cycle. 2004 Jul;3(7):960-7. doi: 10.4161/cc.3.7.1003. Epub 2004 Jul 4.
5
Nutrient starvation promotes condensin loading to maintain rDNA stability.营养饥饿促进凝聚素加载以维持核糖体DNA稳定性。
EMBO J. 2007 Jan 24;26(2):448-58. doi: 10.1038/sj.emboj.7601488. Epub 2007 Jan 4.
6
Unreplicated DNA in mitosis precludes condensin binding and chromosome condensation in S. cerevisiae.有丝分裂中未复制的DNA会阻止酿酒酵母中凝聚素的结合和染色体凝聚。
Front Biosci. 2008 May 1;13:5838-46. doi: 10.2741/3120.
7
The condensin complex governs chromosome condensation and mitotic transmission of rDNA.凝缩蛋白复合体调控染色体凝聚以及核糖体DNA的有丝分裂传递。
J Cell Biol. 2000 May 15;149(4):811-24. doi: 10.1083/jcb.149.4.811.
8
Condensin suppresses recombination and regulates double-strand break processing at the repetitive ribosomal DNA array to ensure proper chromosome segregation during meiosis in budding yeast.凝聚素抑制重复核糖体DNA阵列处的重组并调节双链断裂处理，以确保芽殖酵母减数分裂期间染色体的正确分离。
Mol Biol Cell. 2014 Oct 1;25(19):2934-47. doi: 10.1091/mbc.E14-05-0957. Epub 2014 Aug 7.
9
Compacting DNA during the interphase: condensin maintains rDNA integrity.间期压缩DNA：凝缩蛋白维持核糖体DNA完整性。
Cell Cycle. 2007 Sep 15;6(18):2213-8. doi: 10.4161/cc.6.18.4733. Epub 2007 Jul 11.
10
Condensin function at centromere chromatin facilitates proper kinetochore tension and ensures correct mitotic segregation of sister chromatids.凝聚素在着丝粒染色质处的功能有助于产生适当的动粒张力，并确保姐妹染色单体的正确有丝分裂分离。
Genes Cells. 2007 Sep;12(9):1075-90. doi: 10.1111/j.1365-2443.2007.01109.x.
引用本文的文献
1
Phenotypic tolerance for rDNA copy number variation within the natural range of C. elegans.秀丽隐杆线虫自然范围内rDNA拷贝数变异的表型耐受性。
PLoS Genet. 2025 Jul 2;21(7):e1011759. doi: 10.1371/journal.pgen.1011759. eCollection 2025 Jul.
2
A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace.酵母有丝分裂的故事——核与液泡的拥抱。
Int J Mol Sci. 2023 Jun 6;24(12):9829. doi: 10.3390/ijms24129829.
3
Nucleolar Organizer Regions as Transcription-Based Scaffolds of Nucleolar Structure and Function.核仁组织者区域作为核仁结构和功能的基于转录的支架。
Results Probl Cell Differ. 2022;70:551-580. doi: 10.1007/978-3-031-06573-6_19.
4
The Multiple Roles of the Cdc14 Phosphatase in Cell Cycle Control.Cdc14 磷酸酶在细胞周期调控中的多重作用。
Int J Mol Sci. 2020 Jan 21;21(3):709. doi: 10.3390/ijms21030709.
5
Condensin locates at transcriptional termination sites in mitosis, possibly releasing mitotic transcripts.在有丝分裂中，凝聚素定位于转录终止位点，可能释放有丝分裂转录本。
Open Biol. 2019 Oct 31;9(10):190125. doi: 10.1098/rsob.190125. Epub 2019 Oct 16.
6
Nucleolar and Ribosomal DNA Structure under Stress: Yeast Lessons for Aging and Cancer.应激状态下核仁与核糖体 DNA 结构：衰老与癌症的酵母研究启示
Cells. 2019 Jul 26;8(8):779. doi: 10.3390/cells8080779.
7
Condensin action and compaction.凝缩蛋白的作用与压缩
Curr Genet. 2019 Apr;65(2):407-415. doi: 10.1007/s00294-018-0899-4. Epub 2018 Oct 25.
8
A novel TPR-BEN domain interaction mediates PICH-BEND3 association.一种新型的TPR-BEN结构域相互作用介导了PICH与BEND3的结合。
Nucleic Acids Res. 2017 Nov 2;45(19):11413-11424. doi: 10.1093/nar/gkx792.
9
Heterozygous De Novo UBTF Gain-of-Function Variant Is Associated with Neurodegeneration in Childhood.杂合性新生UBTF功能获得性变异与儿童期神经退行性变相关。
Am J Hum Genet. 2017 Aug 3;101(2):267-273. doi: 10.1016/j.ajhg.2017.07.002.
10
Temperature-dependent regulation of rDNA condensation in Saccharomyces cerevisiae.酿酒酵母中核糖体DNA凝聚的温度依赖性调控
Cell Cycle. 2017 Jun 3;16(11):1118-1127. doi: 10.1080/15384101.2017.1317409. Epub 2017 Apr 20.
本文引用的文献
1
Diverse mitotic and interphase functions of condensins in Drosophila.果蝇中凝缩蛋白的多种有丝分裂和间期功能。
Genetics. 2006 Feb;172(2):991-1008. doi: 10.1534/genetics.105.050567. Epub 2005 Nov 4.
2
FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the Saccharomyces cerevisiae ribosomal DNA locus.FOB1影响酿酒酵母核糖体DNA基因座增强子区域的DNA拓扑异构酶I体内切割。
Nucleic Acids Res. 2005 Nov 3;33(19):6327-37. doi: 10.1093/nar/gki950. Print 2005.
3
Identifying gene-independent noncoding functional elements in the yeast ribosomal DNA by phylogenetic footprinting.通过系统发育足迹法鉴定酵母核糖体DNA中与基因无关的非编码功能元件。
Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11787-92. doi: 10.1073/pnas.0504905102. Epub 2005 Aug 4.
4
Condensin binding at distinct and specific chromosomal sites in the Saccharomyces cerevisiae genome.凝缩蛋白在酿酒酵母基因组中不同且特定的染色体位点上的结合。
Mol Cell Biol. 2005 Aug;25(16):7216-25. doi: 10.1128/MCB.25.16.7216-7225.2005.
5
Condensins: organizing and segregating the genome.凝聚素：组织和分离基因组
Curr Biol. 2005 Apr 12;15(7):R265-75. doi: 10.1016/j.cub.2005.03.037.
6
The expanding TOR signaling network.不断扩展的TOR信号网络。
Curr Opin Cell Biol. 2005 Apr;17(2):158-66. doi: 10.1016/j.ceb.2005.02.008.
7
Spindle-independent condensation-mediated segregation of yeast ribosomal DNA in late anaphase.酵母核糖体DNA在后期通过纺锤体非依赖的凝聚介导的分离。
J Cell Biol. 2005 Jan 17;168(2):209-19. doi: 10.1083/jcb.200408087.
8
rDNA enhancer affects replication initiation and mitotic recombination: Fob1 mediates nucleolytic processing independently of replication.核糖体DNA增强子影响复制起始和有丝分裂重组：Fob1独立于复制介导核酸酶加工。
Mol Cell. 2004 Aug 13;15(3):409-21. doi: 10.1016/j.molcel.2004.06.024.
9
Cdc14p/FEAR pathway controls segregation of nucleolus in S. cerevisiae by facilitating condensin targeting to rDNA chromatin in anaphase.Cdc14p/FEAR途径通过促进后期凝聚素靶向rDNA染色质来控制酿酒酵母中核仁的分离。
Cell Cycle. 2004 Jul;3(7):960-7. doi: 10.4161/cc.3.7.1003. Epub 2004 Jul 4.
10
Spatial and temporal regulation of Condensins I and II in mitotic chromosome assembly in human cells.人类细胞有丝分裂染色体组装过程中凝聚素I和II的时空调控
Mol Biol Cell. 2004 Jul;15(7):3296-308. doi: 10.1091/mbc.e04-03-0242. Epub 2004 May 14.
Zotero 插件