Loukusa A K, Veijola M, Rajaniemi H
Department of Anatomy, University of Oulu, Finland.
Int J Androl. 1990 Aug;13(4):306-14. doi: 10.1111/j.1365-2605.1990.tb01036.x.
The role of proteolytic enzymes in the hCG-induced increase in testicular vasopermeability and neutrophil extravasation was studied using protease inhibitors. An intra-testicular injection of hCG together with incubation medium conditioned by polymorphonuclear leucocytes (PMNs) caused a significant increase in vasopermeability and a coincident extravasation of PMN's from the postcapillary venules in the rat testis. When p-aminobenzamidine, a serine protease inhibitor which inhibits urokinase-type plasminogen activator, was administered together with hCG in the incubation medium, both the permeability increase and PMN extravasation were prevented. Aprotinin, another serine protease inhibitor, and Eglin C, a specific neutrophil elastase and cathepsin G inhibitor were, however, without effect. None of these inhibitors caused any non-specific vascular effects in the testis at the concentrations used. These results support the concept that the hCG-induced increase in vasopermeability in the rat testis is related to extravasation of PMNs and suggest that urokinase-type plasminogen activator is involved in migration of these cells through the postcapillary venular walls.
利用蛋白酶抑制剂研究了蛋白水解酶在人绒毛膜促性腺激素（hCG）诱导的睾丸血管通透性增加和中性粒细胞渗出中的作用。在大鼠睾丸内注射hCG并与多形核白细胞（PMN）条件培养液一起孵育，可导致血管通透性显著增加，同时PMN从毛细血管后微静脉渗出。当在孵育液中与hCG一起给予对氨基苯甲脒（一种抑制尿激酶型纤溶酶原激活物的丝氨酸蛋白酶抑制剂）时，可防止通透性增加和PMN渗出。然而，另一种丝氨酸蛋白酶抑制剂抑肽酶以及一种特异性中性粒细胞弹性蛋白酶和组织蛋白酶G抑制剂依格尔菌素C却没有效果。在所使用的浓度下，这些抑制剂均未在睾丸中引起任何非特异性血管效应。这些结果支持了hCG诱导的大鼠睾丸血管通透性增加与PMN渗出有关的观点，并表明尿激酶型纤溶酶原激活物参与了这些细胞通过毛细血管后微静脉壁的迁移。
