Veijola M, Rajaniemi H
Department of Anatomy, University of Oulu, Finland.
Int J Androl. 1989 Aug;12(4):307-17. doi: 10.1111/j.1365-2605.1989.tb01318.x.
An intratesticular injection of hCG (5 ng) mixed with testicular interstitial fluid (IF) increases vascular permeability in the rat testis. The present results show that the permeability increase induced by this treatment is accompanied by a massive accumulation of polymorphonuclear leucocytes (PMNs) in both the testicular postcapillary venules and the interstitium. Depletion of neutrophils in the circulation by treatment with anti-neutrophil serum significantly inhibited the permeability increase induced by this treatment. An intratesticular injection of PMNs (10(7) cells) or hCG alone had no effect on permeability, but a combination of the two caused a significant increase in permeability. The PMNs were found to secrete a component in vitro which, when injected intratesticularly together with hCG, caused a increase and a simultaneous massive accumulation of PMNs in the postcapillary venules and interstitium. This permeability increase was prevented by the serine protease inhibitor p-aminobenzamidine, suggesting an involvement of the plasminogen activator system in the response. The results suggest that hCG interacts with an IF component to produce leucotactic factors that increase permeability indirectly by attracting PMNs to the tissue, and that the IF component may originate in the PMNs.
将5纳克人绒毛膜促性腺激素（hCG）与睾丸间质液（IF）混合后进行睾丸内注射，可增加大鼠睾丸的血管通透性。目前的结果表明，这种处理诱导的通透性增加伴随着多形核白细胞（PMN）在睾丸毛细血管后微静脉和间质中的大量积聚。用抗中性粒细胞血清处理使循环中的中性粒细胞耗竭，可显著抑制这种处理诱导的通透性增加。单独进行睾丸内注射PMN（10⁷个细胞）或hCG对通透性没有影响，但两者联合使用会导致通透性显著增加。发现PMN在体外分泌一种成分，当与hCG一起进行睾丸内注射时，会导致毛细血管后微静脉和间质中的通透性增加以及PMN同时大量积聚。丝氨酸蛋白酶抑制剂对氨基苯甲脒可阻止这种通透性增加，提示纤溶酶原激活系统参与了该反应。结果表明，hCG与一种IF成分相互作用产生趋白细胞因子，通过将PMN吸引到组织中间接增加通透性，并且该IF成分可能起源于PMN。
