Tapanainen J M, Paloneva T, Veijola M, Rajaniemi H
Department of Anatomy, University of Oulu, Finland.
J Reprod Fertil. 1990 Jul;89(2):723-8. doi: 10.1530/jrf.0.0890723.
An intradermal injection of testicular interstitial fluid (IF) produced a marked increase in vasopermeability in a dose-dependent manner. Likewise bovine follicular fluid caused a smaller but significant response. The effect of IF was associated with accumulation of polymorphonuclear leucocytes (PMNs) inside the dermal venules and with their adherence to the venular endothelium. A minor but significant response was noticed after injecting anterior chamber fluid, but there was no response after an injection of amniotic fluid or serum intracutaneously. Destroying the Leydig cells with ethane dimethanesulphonate did not change the vasopermeability-increasing effect of IF, but after denaturation of IF proteins the effect was diminished by about 50%. Intravenous administration of hCG did not increase the ability of IF to cause the effect. These results suggest that rat testicular interstitial fluid contains mediators of vasopermeability, probably specific for the testis and also follicular fluid. The vasopermeability effect of IF does not seem to depend on the collecting time or on Leydig cells and is at least partly mediated by PMNs which are seen in the dermal venules shortly after an injection of IF.
皮内注射睾丸间质液（IF）可使血管通透性以剂量依赖方式显著增加。同样，牛卵泡液也会引起较小但显著的反应。IF的作用与多形核白细胞（PMN）在真皮小静脉内的积聚及其对小静脉内皮的黏附有关。注射前房液后观察到轻微但显著的反应，但皮内注射羊水或血清后无反应。用乙烷二甲磺酸盐破坏睾丸间质细胞并不会改变IF增加血管通透性的作用，但IF蛋白变性后，该作用会减弱约50%。静脉注射人绒毛膜促性腺激素（hCG）并不会增强IF产生该效应的能力。这些结果表明，大鼠睾丸间质液含有血管通透性介质，可能对睾丸具有特异性，卵泡液中也含有。IF的血管通透性效应似乎不取决于采集时间或睾丸间质细胞，并且至少部分由注射IF后不久在真皮小静脉中可见的PMN介导。
