Kamel A M, Ghaleb F M, Assem M M, Hindawy D S, Jaffe E S, Magrath I T
Department of Clinical Pathology, National Cancer Institute, Cairo University, Egypt.
Leuk Res. 1990;14(7):601-9. doi: 10.1016/0145-2126(90)90014-z.
Leukemic cells from 46 T ALL cases were studied with a wide panel of mAb reacting with T cells using an immunoperoxidase technique. The cases included 15 adults (16 years or over) and 31 children (less than 16 years). The mAb used in the panel were: CD1, (T6), CD2 (T11, X11, D66, clone 2), CD3 (T3/Leu4), CD4, (T4/Leu3a), CD5 (Leu1, T1, A50, I73D9), CD7 (Leu9, I21), CD8 (T8/Leu2a) and HNK1. Based on their reactivity with the mAb panel all cases were assigned to one of the intrathymic differentiation compartments. Among the adults, five cases were assigned to compartment I, six to compartment II and four to compartment III. The pediatric cases included eight in compartment I, eighteen in compartment II and five in compartment III. Fifteen L1 cases studied included four in compartment I, seven in compartment II and four in compartment III; while thirty L2 cases showed nine in compartment I, sixteen in compartment II and five cases in compartment III. The most frequently observed CD groups among T ALL cells were CD5 (100% and 88.9% in children and adults respectively), CD7 (93.1% and 84.6%) and CD2 (76.7% and 76.9%). The most frequently reactive mAb in our series was Leu1 (81.8% followed by I21 (71.1%) and Leu9 (68.9%). The most frequently reactive combination of two mAb was Leu1/I21 (100%) followed by Leu1/T11 (97.4%). Five of the 46 cases reacted with HNK1, suggesting an origin from the natural killer (NK) subset. Our results indicate that in T-cell ALL in Egypt the surface phenotype is similar to that of intermediate or late thymocytes in more than 66% of cases. CD5, CD7 and CD2 were the most frequently detected antigens. Studies of the association between T-cell phenotype and socioeconomic status are warranted.
采用免疫过氧化物酶技术，用一组与T细胞反应的单克隆抗体（mAb）对46例T细胞急性淋巴细胞白血病（T ALL）病例的白血病细胞进行了研究。这些病例包括15名成人（16岁及以上）和31名儿童（小于16岁）。该组使用的单克隆抗体有：CD1（T6）、CD2（T11、X11、D66、克隆2）、CD3（T3/Leu4）、CD4（T4/Leu3a）、CD5（Leu1、T1、A50、I73D9）、CD7（Leu9、I21）、CD8（T8/Leu2a）和HNK1。根据它们与该组单克隆抗体的反应性，所有病例被归入胸腺内分化区之一。在成人中，5例被归入I区，6例归入II区，4例归入III区。儿童病例中，I区有8例，II区有18例，III区有5例。研究的15例L1病例中，I区有4例，II区有7例，III区有4例；而30例L2病例中，I区有9例，II区有16例，III区有5例。T ALL细胞中最常观察到的CD组是CD5（儿童和成人中分别为100%和88.9%）、CD7（93.1%和84.6%）和CD2（76.7%和76.9%）。我们系列中最常反应的单克隆抗体是Leu1（81.8%），其次是I21（71.1%）和Leu9（68.9%）。两种单克隆抗体最常反应的组合是Leu1/I21（100%），其次是Leu1/T11（97.4%）。46例病例中有5例与HNK1反应，提示起源于自然杀伤（NK）亚群。我们的结果表明，在埃及的T细胞急性淋巴细胞白血病中，超过66%的病例表面表型与中晚期胸腺细胞相似。CD5、CD7和CD2是最常检测到的抗原。有必要研究T细胞表型与社会经济地位之间的关联。
