Solínová Veronika, Kasicka Václav
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
J Sep Sci. 2006 Aug;29(12):1743-62. doi: 10.1002/jssc.200600167.
The review provides a comprehensive survey of the recent applications of contact and contactless conductivity detection in capillary electrophoretic and chip electrophoretic analyses of a broad scale of compounds, from low-molecular-mass highly mobile small inorganic and organic ions, via medium-molecular-mass peptides and oligo- and polynucleotides up to high-molecular-mass biopolymers, proteins and nucleic acids fragments. The review presents also the recent developments in the construction of different types of conductivity detectors (detectors with galvanic contact of the sensing electrodes with the BGE and sample components, contactless conductivity detectors with capacitively coupled tubular and semitubular electrodes and combined conductivity/optical detectors) applied in the capillary electromigration methods performed in classical fused silica, polytetrafluorethylene, and polyetheretherketone capillaries or on glass and polymethylmethacrylate microchips. In addition, the principle and theoretical bases of conductivity detection in capillary electromigration techniques, zone electrophoresis, ITP, micellar EKC, and electrochromatography are briefly described.
本综述全面概述了接触式和非接触式电导检测在毛细管电泳和芯片电泳分析中的最新应用，这些分析涉及广泛的化合物，从低分子量、高迁移率的无机和有机小分子离子，到中等分子量的肽、寡核苷酸和多核苷酸，直至高分子量的生物聚合物、蛋白质和核酸片段。本综述还介绍了不同类型电导检测器（传感电极与缓冲液和样品组分进行电流接触的检测器、具有电容耦合管状和半管状电极的非接触式电导检测器以及组合式电导/光学检测器）的最新进展，这些检测器应用于在传统熔融石英、聚四氟乙烯和聚醚醚酮毛细管中或玻璃和聚甲基丙烯酸甲酯微芯片上进行的毛细管电迁移方法。此外，还简要描述了毛细管电迁移技术、区带电泳、等速电泳、胶束电动色谱和电色谱中电导检测的原理和理论基础。
