Ege University, Faculty of Pharmacy, Analytical Chemistry Department, Bornova, 35100 Izmir, Turkey.
Analyst. 2011 Mar 7;136(5):1041-5. doi: 10.1039/c0an00357c. Epub 2011 Jan 4.
The development of amino-terminated G4 PAMAM dendrimer (PDR) modified disposable electrodes were developed as the first time in our study by using the dendrimer modified disposable graphite (PDR-PGE) and multiwalled carbon nanotube based screen-printed graphite (PDR-MWCNT-SPE) electrodes. Firstly, the microscopic characterization of bare PGEs and PDR modified PGEs was performed. These sensors were then applied for electrochemical monitoring of an anticancer drug, Daunorubicin (DNR). The enhanced oxidation signal of DNR was measured at +0.50 V by using differential pulse voltammetry (DPV) in combination with the PDR-PGEs. The detection limit, estimated from S/N = 3, corresponds accordingly to 317 nM and 128 nM for DNR respectively at the PGE and PDR-PGE. The voltammetric results were consistent with electrochemical impedance spectroscopy (EIS) that was used to characterize the successful modification of PDR onto the surface of PGE and MWCNT-SPE.
本研究首次开发了末端氨基化 G4 PAMAM 树枝状聚合物（PDR）修饰的一次性电极，通过使用树枝状聚合物修饰的一次性石墨（PDR-PGE）和基于多壁碳纳米管的丝网印刷石墨（PDR-MWCNT-SPE）电极。首先，对裸 PGE 和 PDR 修饰的 PGE 进行了微观特征描述。然后，这些传感器被用于电化学监测抗癌药物柔红霉素（DNR）。通过差分脉冲伏安法（DPV）与 PDR-PGE 相结合，在+0.50 V 处测量了 DNR 的增强氧化信号。根据 S/N = 3 计算出的检测限，分别对应于 PGE 和 PDR-PGE 处的 317 nM 和 128 nM。伏安法结果与电化学阻抗谱（EIS）一致，EIS 用于表征 PDR 成功修饰到 PGE 和 MWCNT-SPE 表面。
