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基于iTRAQ的蛋白质组学分析揭示马铃薯冷胁迫响应中的分子途径。

iTRAQ-Based Proteomic Analysis Reveals Molecular Pathways in Potato Cold Stress Response.

作者信息

Yang Huiju, Chen Mingwei, Guo Huachun

机构信息

Lijiang Normal University, Lijiang 674199, China.

College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650205, China.

出版信息

Life (Basel). 2025 May 30;15(6):885. doi: 10.3390/life15060885.

DOI:10.3390/life15060885
PMID:40566539
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12194501/
Abstract

Potato ( L.) is an essential crop for food and industrial production, but its cultivation in low-temperature regions is challenging. Therefore, using proteomics quantification technology based on isobaric tags for relative and absolute quantification, we investigated molecular responses in potato leaves under cold stress at 1 (L1), 3 (L3), and 6 h (L6), with normal growth as a control. A total of 3292 proteins at all stages were identified, with 125, 250, and 380 differentially abundant proteins at L1, L3, and L6, respectively. The GO and KEGG analysis revealed that these DAPs were linked to photosynthesis, oxidative phosphorylation, and the biosynthesis of ansamycins. Further association analysis showed down-regulation in aminoacyl-tRNA biosynthesis and up-regulation in pathways like alpha-linolenic acid metabolism. At L6, a significant up-regulation of cold tolerance genes was observed. This study provides insight into the proteomic mechanisms of cold tolerance in potato, laying a foundation for genetic breeding.

摘要

马铃薯（茄属）是粮食和工业生产的重要作物，但其在低温地区的种植具有挑战性。因此，我们使用基于等压标签相对和绝对定量的蛋白质组学定量技术，以正常生长为对照，研究了马铃薯叶片在1小时（L1）、3小时（L3）和6小时（L6）冷胁迫下的分子反应。共鉴定出各阶段的3292种蛋白质，在L1、L3和L6分别有125、250和380种差异丰富蛋白质。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，这些差异丰富蛋白质与光合作用、氧化磷酸化和安莎霉素生物合成有关。进一步的关联分析显示，氨酰-tRNA生物合成下调，而α-亚麻酸代谢等途径上调。在L6时，观察到耐寒基因显著上调。本研究深入了解了马铃薯耐寒性的蛋白质组学机制，为遗传育种奠定了基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d4/12194501/a2924b5f342f/life-15-00885-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d4/12194501/f5de2376cb99/life-15-00885-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d4/12194501/a2924b5f342f/life-15-00885-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d4/12194501/1de9cdd9f0d7/life-15-00885-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d4/12194501/a6dc13b7042e/life-15-00885-g002.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d4/12194501/7895a7686c4f/life-15-00885-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b9d4/12194501/28b6ce018b79/life-15-00885-g005.jpg
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