Singh Shriya, Hegde Manasa, Kaur Inderjeet, Adlakha Nidhi
Synthetic Biology and Bioprocessing Group, Regional Centre for Biotechnology, NCR-Biotech Cluster, Faridabad, Haryana, India.
Department of Biotechnology, Central University of Haryana, Mahendergarh, Haryana, India.
Sci Rep. 2025 Apr 7;15(1):11857. doi: 10.1038/s41598-025-92683-5.
Botrytis cinerea is a necrotrophic fungal pathogen that poses a significant threat to many crops. Understanding the proteome dynamics of phytopathogens during infection can help combat plant diseases. However, most proteomics studies in phytopathogens face interference from abundant host proteins. Here, we optimized a solid media that better mimics in-planta conditions and used it to perform the temporal protein dynamics in Botrytis cinerea. An agar media with 20% tomato fruit extract and 2% deproteinised leaf extract was utilized for label-free quantitative proteomics at 12, 36, 72 and 120 hpi. Out of 3244 quantified proteins, 2045 showed differential regulation. Glycosyl hydrolases, pectin esterases, stress protein DDR48, RhoGEF and essential transcription factors were found to be upregulated during the early phase, highlighting their role in fungal virulence. Meanwhile, pathways such as macromolecule synthesis, purine, and carbohydrate metabolism were upregulated in the late-growth phase. Overall, the study provides a comprehensive understanding of proteome dynamics during Botrytis infection.
灰葡萄孢是一种坏死营养型真菌病原体，对许多作物构成重大威胁。了解植物病原体在感染过程中的蛋白质组动态有助于防治植物病害。然而，大多数针对植物病原体的蛋白质组学研究都面临来自丰富宿主蛋白的干扰。在此，我们优化了一种能更好模拟植物体内条件的固体培养基，并利用它来研究灰葡萄孢的时间蛋白质动态。一种含有20%番茄果实提取物和2%脱蛋白叶片提取物的琼脂培养基被用于在接种后12、36、72和120小时进行无标记定量蛋白质组学研究。在3244种定量蛋白质中，有2045种表现出差异调节。糖基水解酶、果胶酯酶、应激蛋白DDR48、Rho鸟嘌呤核苷酸交换因子和必需转录因子在早期阶段被上调，突出了它们在真菌毒力中的作用。同时，大分子合成、嘌呤和碳水化合物代谢等途径在生长后期被上调。总体而言，该研究全面了解了灰葡萄孢感染过程中的蛋白质组动态。
