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拟南芥种子的定量相位断层扫描揭示细胞间空隙网络。

Quantitative phase tomography of Arabidopsis seeds reveals intercellular void network.

作者信息

Cloetens Peter, Mache Régis, Schlenker Michel, Lerbs-Mache Silva

机构信息

European Synchrotron Radiation Facility, B.P. 220, F-38043 Grenoble, France.

出版信息

Proc Natl Acad Sci U S A. 2006 Sep 26;103(39):14626-30. doi: 10.1073/pnas.0603490103. Epub 2006 Sep 14.

DOI:10.1073/pnas.0603490103
PMID:16973748
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1600010/
Abstract

We used quantitative phase tomography with synchrotron radiation to elucidate the 3D structure of Arabidopsis seeds in their native state. The cells are clearly distinguished, and their internal structure is revealed through local variations in electron density. We visualized a 3D network of intercellular air space that might allow immediate gas exchange for energy supply during germination and/or serve for rapid water uptake and distribution during imbibition.

摘要

我们使用同步辐射定量相断层扫描技术来阐明拟南芥种子在其自然状态下的三维结构。细胞清晰可辨,其内部结构通过电子密度的局部变化得以揭示。我们可视化了细胞间气腔的三维网络,这可能在种子萌发期间允许即时的气体交换以供应能量,和/或在吸水过程中用于快速的水分吸收和分布。

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本文引用的文献

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Variable timing of developmental progression in the stomatal pathway in Arabidopsis cotyledons.拟南芥子叶气孔发育途径中发育进程的可变时间
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Water uptake and distribution in germinating tobacco seeds investigated in vivo by nuclear magnetic resonance imaging.利用核磁共振成像技术在活体中研究萌发烟草种子的水分吸收与分布情况。
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X-ray tomography generates 3-D reconstructions of the yeast, saccharomyces cerevisiae, at 60-nm resolution.X射线断层扫描以60纳米的分辨率生成酿酒酵母的三维重建图像。
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Energy status and its control on embryogenesis of legumes. Embryo photosynthesis contributes to oxygen supply and is coupled to biosynthetic fluxes.能量状态及其对豆科植物胚胎发生的控制。胚胎光合作用有助于氧气供应,并与生物合成通量相关联。
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Protoxylem: the deposition of a network containing glycine-rich cell wall proteins starts in the cell corners in close association with the pectins of the middle lamella.原生木质部:富含甘氨酸的细胞壁蛋白网络的沉积始于细胞角隅,与中层果胶紧密相连。
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