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通过同步辐射X射线成像对大豆根瘤功能结构进行可视化和定量评估

Visualization and Quantitative Evaluation of Functional Structures of Soybean Root Nodules via Synchrotron X-ray Imaging.

作者信息

Nakhforoosh Alireza, Hallin Emil, Karunakaran Chithra, Korbas Malgorzata, Stobbs Jarvis, Kochian Leon

机构信息

Global Institute for Food Security, Saskatoon, SK S7N 4L8, Canada.

Canadian Light Source Inc., Saskatoon, SK S7N 2V3, Canada.

出版信息

Plant Phenomics. 2024 Jul 17;6:0203. doi: 10.34133/plantphenomics.0203. eCollection 2024.

DOI:10.34133/plantphenomics.0203
PMID:39021394
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11254386/
Abstract

The efficiency of N-fixation in legume-rhizobia symbiosis is a function of root nodule activity. Nodules consist of 2 functionally important tissues: (a) a central infected zone (CIZ), colonized by rhizobia bacteria, which serves as the site of N-fixation, and (b) vascular bundles (VBs), serving as conduits for the transport of water, nutrients, and fixed nitrogen compounds between the nodules and plant. A quantitative evaluation of these tissues is essential to unravel their functional importance in N-fixation. Employing synchrotron-based x-ray microcomputed tomography (SR-μCT) at submicron resolutions, we obtained high-quality tomograms of fresh soybean root nodules in a non-invasive manner. A semi-automated segmentation algorithm was employed to generate 3-dimensional (3D) models of the internal root nodule structure of the CIZ and VBs, and their volumes were quantified based on the reconstructed 3D structures. Furthermore, synchrotron x-ray fluorescence imaging revealed a distinctive localization of Fe within CIZ tissue and Zn within VBs, allowing for their visualization in 2 dimensions. This study represents a pioneer application of the SR-μCT technique for volumetric quantification of CIZ and VB tissues in fresh, intact soybean root nodules. The proposed methods enable the exploitation of root nodule's anatomical features as novel traits in breeding, aiming to enhance N-fixation through improved root nodule activity.

摘要

豆科植物与根瘤菌共生体系中的固氮效率是根瘤活性的函数。根瘤由两个功能重要的组织组成：(a) 中央感染区（CIZ），被根瘤菌定殖，是固氮的场所；(b) 维管束（VBs），作为根瘤与植物之间水分、养分和固定氮化合物运输的管道。对这些组织进行定量评估对于阐明它们在固氮中的功能重要性至关重要。我们利用基于同步加速器的亚微米分辨率X射线显微计算机断层扫描（SR-μCT），以非侵入性方式获得了新鲜大豆根瘤的高质量断层图像。采用半自动分割算法生成了CIZ和VBs内部根瘤结构的三维（3D）模型，并根据重建的3D结构对其体积进行了量化。此外，同步加速器X射线荧光成像揭示了Fe在CIZ组织中的独特定位以及Zn在VBs中的独特定位，从而实现了它们在二维中的可视化。本研究代表了SR-μCT技术在新鲜完整大豆根瘤中对CIZ和VB组织进行体积定量的开创性应用。所提出的方法能够利用根瘤的解剖特征作为育种中的新性状，旨在通过提高根瘤活性来增强固氮作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/0b1168ac9006/plantphenomics.0203.fig.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/78a059d13146/plantphenomics.0203.fig.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/448896aeb99d/plantphenomics.0203.fig.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/e858d9bd36b0/plantphenomics.0203.fig.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/6c011e7e0029/plantphenomics.0203.fig.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/30b8e0578e88/plantphenomics.0203.fig.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/59c802b23312/plantphenomics.0203.fig.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/a6c1d80b75df/plantphenomics.0203.fig.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/a76f26472256/plantphenomics.0203.fig.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/0b1168ac9006/plantphenomics.0203.fig.009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/78a059d13146/plantphenomics.0203.fig.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/448896aeb99d/plantphenomics.0203.fig.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/e858d9bd36b0/plantphenomics.0203.fig.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/6c011e7e0029/plantphenomics.0203.fig.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/30b8e0578e88/plantphenomics.0203.fig.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/59c802b23312/plantphenomics.0203.fig.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/a6c1d80b75df/plantphenomics.0203.fig.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/a76f26472256/plantphenomics.0203.fig.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d6e/11254386/0b1168ac9006/plantphenomics.0203.fig.009.jpg

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