Shapiro David, Thibault Pierre, Beetz Tobias, Elser Veit, Howells Malcolm, Jacobsen Chris, Kirz Janos, Lima Enju, Miao Huijie, Neiman Aaron M, Sayre David
Department of Physics and Astronomy, Stony Brook University, Stony Brook, NY 11794-3800, USA.
Proc Natl Acad Sci U S A. 2005 Oct 25;102(43):15343-6. doi: 10.1073/pnas.0503305102. Epub 2005 Oct 11.
We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the accuracy of the imaging technique. To obtain the best possible reconstructions, we have implemented procedures for handling noisy and incomplete diffraction data, and we propose a method for determining the reconstructed resolution. This work represents a previously uncharacterized application of x-ray diffraction microscopy to a specimen of this complexity and provides confidence in the feasibility of the ultimate goal of imaging biological specimens at 10-nm resolution in three dimensions.
我们采用X射线衍射显微镜方法对完整且未染色的酵母细胞的复值出射波进行成像。通过使用来自不同角度方向的750电子伏特X射线获得的冻干细胞图像，以30纳米的分辨率描绘了细胞的几个主要内部成分。独立恢复的结构之间的良好一致性证明了成像技术的准确性。为了获得尽可能好的重建结果，我们实施了处理噪声和不完整衍射数据的程序，并提出了一种确定重建分辨率的方法。这项工作代表了X射线衍射显微镜在这种复杂标本上以前未被表征的应用，并为在三维空间中以10纳米分辨率对生物标本进行成像这一最终目标的可行性提供了信心。
